短链脂肪酸盐对小鼠腹腔巨噬细胞的抗炎功能研究  

Anti-inflammatory effect of short-chain fatty acid salt on the mouse peritoneal macrophages

作　　者：刘鹏[1] 姚梦 李志恒 潘万龙[1] LIU Peng;YAO Meng;LI Zhi-heng;PAN Wan-long(College of Basic Medicine and Forensic Medicine,North Sichuan Medical College;Department of Clinical Laboratory,Nanchong Central Hospital,Nanchong 637000,Sichuan,China)

机构地区：[1]川北医学院基础医学与法医学院 [2]南充市中心医院检验科,四川南充637000

出　　处：《川北医学院学报》2024年第2期156-160,共5页Journal of North Sichuan Medical College

基　　金：四川省南充市市校合作项目(19SXHZ0134);川北医学院校级项目(CBY22-QNA52)。

摘　　要：目的:探讨短链脂肪酸盐中丙酸钠与丁酸钠对小鼠腹腔巨噬细胞代谢活性、一氧化氮(NO)、炎性细胞因子[白细胞介素1(IL-1)、IL-6及肿瘤坏死因子α(TNF-α)]分泌及吞噬杀菌功能的影响。方法:采用CCK-8检测SCFAs处理后巨噬细胞代谢活性;Griess试剂与ELISA法分别检测SCFAs干预经LPS刺激的巨噬细胞上清液中NO与炎性细胞因子;巨噬细胞吞噬适宜浓度鼠伤寒沙门菌后,经SCFAs干预,平板稀释计数法检测吞噬0、5、10 h细菌存活数,同时检测各组10 h上清液中NO含量。结果:丙酸钠与丁酸钠均能降低巨噬细胞代谢活性,降低LPS刺激后NO、IL-6及TNF-α水平,使巨噬细胞吞噬鼠伤寒沙门菌5 h与10 h后的胞内细菌数量增加。结论:丙酸钠与丁酸钠能降低由LPS诱导的小鼠腹腔巨噬细胞炎症反应,增加鼠伤寒沙门菌在巨噬细胞内的存活机率。Objective:To study the effects of sodium propionate and sodium butyrate in short-chain fatty acid salts on metabolic activity,nitric oxide(NO),inflammatory cytokines[interleukin-1(IL-1),IL-6,and tumor necrosis factorα(TNF-α)]and phagocytic bactericidal function of mouse peritoneal macrophages.Methods:CCK-8 was used to detect the metabolic activity of macrophages after SCFAs treatment.Griess reagent and ELISA were used to detect NO and inflammatory cytokines in the supernatant of macrophages stimulated by SCFAs.After phagocytosis of suitable concentration of Salmonella typhimurium,the amount of NO in the superserum was detected by SCFAs at 10 h time points,and the survival number of bacteria at 0,5,and 10 h phagocytosis time points was detected by plate dilution counting methods.Results:Both sodium propionate and sodium butyrate could decrease the metabolic activity of macrophages and the levels of NO and inflammatory cytokines IL-6 and TNF-αafter LPS stimulation.The number of intracellular bacteria increased after macrophages phagocytosis of Salmonella typhimurium at 5 and 10 h.Conclusion:Sodium propionate and sodium butyrate can reduce the inflammatory response of peritoneal macrophages induced by LPS and increase the survival rate of Salmonella typhimurium in macrophages.

关 键 词：短链脂肪酸盐 鼠伤寒沙门菌 腹腔巨噬细胞 抗炎作用 

分 类 号：R392.12[医药卫生—免疫学]