肿瘤坏死因子样细胞凋亡弱诱导物/成纤维细胞生长因子诱导早期反应蛋白14轴及下游核因子κB通路的关键因子mRNA及蛋白表达差异与老年肥胖的相关性  

Correlation between obesity and difference of mRNA and protein expression of key factors of tumor necrosis factor-like weak inducer of apoptosis/fibroblast growth factor-induced early reactive protein 14 and downstream nuclear factor-kappa B pathway in the elderly

作　　者：赵艳姣 卓娅·买买提乌斯满[1] 刘金玲 王红梅[1] Zhao Yanjiao;Zhuoya·Maimaitiwusiman;Liu Jinling;Wang Hongmei(Second Department of Cadre Health Care Center,People's Hospital of Xinjiang Uygur Autonomous Region,Urumqi 830000,China)

机构地区：[1]新疆维吾尔自治区人民医院综合保健内科二病区,乌鲁木齐830000

出　　处：《中华老年多器官疾病杂志》2024年第2期81-86,共6页Chinese Journal of Multiple Organ Diseases in the Elderly

基　　金：国家自然科学基金(82160276);新疆维吾尔自治区自然科学基金(2021D01C162);新疆维吾尔自治区人民医院院内项目(20200207)。

摘　　要：目的研究老年人外周血单个核细胞(PBMC)中肿瘤坏死因子样细胞凋亡弱诱导物(TWEAK)/成纤维细胞生长因子诱导早期反应蛋白14(Fn14)轴及下游核因子κB(NF-κB)通路的关键因子mRNA及蛋白表达差异与老年人中心性肥胖的相关性。方法2017年9月至2018年5月在新疆农牧区常住居民横断面流行病学调查数据库中随机抽取80例研究对象,根据是否为中心性肥胖分为对照组(n=40)和中心性肥胖组(n=40),提取空腹外周血中PBMC,采用待实时荧光定量聚合酶链反应、Western blotting方法检测TWEAK、Fn14、IκB激酶α(IKKα)、IκB激酶β(IKKβ)、NF-κBp65的mRNA、蛋白表达。采用SPSS 26.0软件进行数据分析。根据数据类型,组间比较分别采用t检验及χ2检验。相关性分析采用双变量Pearson相关分析法。结果中心性肥胖组外周血PBMC中的Fn14、IKKα、IKKβ的mRNA表达高于对照组(P<0.05);TWEAK、NF-κBp65的mRNA表达也高于对照组,但差异无统计学意义(P>0.05)。中心性肥胖组的Fn14、IKKα、IKKβ蛋白表达水平高于对照组(P<0.05),两组间TWEAK、NF-κBp65蛋白表达水平差异无统计学意义(P>0.05)。双变量Pearson相关性分析显示,上述因子的mRNA表达水平,TWEAK与Fn14呈正相关(r=0.472;P<0.01),Fn14与IKKα、IKKβ均呈正相关(r=0.262,0.275;P<0.05);IKKα、IKKβ与NF-κBp65均呈正相关(r=0.747,0.692;P<0.01)。结论新疆农牧区常驻老年人中心性肥胖与外周血PBMC中Fn14、IKKα、IKKβ的蛋白、mRNA的高表达相关,TWEAK/Fn14可能通过调节IKK激活NF-κB炎症通路,参与人类肥胖的发生、发展。Objective To study the correlation between central obesity and the difference of mRNA and protein expression of the tumor necrosis factor-like weak inducer of apoptosis(TWEAK)/fibroblast growth factor-induced early reactive protein 14(Fn14)and downstream nuclear factor-kappa B(NF-κB)pathway in peripheral blood mononuclear cells(PBMC)in the elderly.Methods From September 2017 to May 2018,80 subjects were randomly selected from the database of cross-sectional epidemiological survey of permanent residents in agricultural and pastoral areas of Xinjiang.According to whether they had central obesity or not,they were divided into the control group(n=40)and the central obesity group(n=40).PBMC were extracted from fasting peripheral blood,and the mRNA and protein expressions of TWEAK,Fn14,inhibitor of kappa B kinase-α(IKKα),inhibitor of kappa B kinase-β(IKKβ)and NF-κBp65 were detected by quantitative real-time polymerase chain reaction and Western-blotting.SPSS 26.0 was used for statistical analysis.Data comparison between two groups was performed using t-test orχ2 test,depending on data type.Correlation was analyzed using bivariate Pearson correlation analysis method.Results The mRNA expression of Fn14,IKKα,IKKβ,TWEAK and NF-κBp65 in PBMC in the central obesity group was higher than that in the control group,but the difference was not statistically significant(P>0.05).The protein expression levels of Fn14,IKKαand IKKβin the central obesity group were higher than those in the control group(P<0.05),but there was no significant difference in TWEAK and NF-κBp65 protein expression between the two groups(P>0.05).Bivariate Pearson correlation analysis showed that mRNA expression of the above factors was positively correlated with TWEAK and Fn14(r=0.472;P<0.01),Fn14 was positively correlated with IKKαand IKKβ(r=0.262,0.275;P<0.05),and that IKKαand IKKβwere positively correlated with NF-κBp65(r=0.747,0.692;P<0.01).Conclusion Central obesity in the elderly in the agricultural and pastoral areas of Xinjiang is rel

关 键 词：老年人 中心性肥胖 TWEAK Fn14 NF-ΚB通路 

分 类 号：R589.2[医药卫生—内分泌]