贵州辣椒番茄斑萎病毒的分子鉴定  

Molecular Detection of Tomato Spotted Wilt Virus Infected Pepper in Guizhou

作　　者：田雨 李淳 李岩 王莉爽 TIAN Yu;LI Chun;LI Yan;WANG Lishuang(Key laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region(Ministry of Education),College of Life Sciences,Institute of Agro-bioengineering,Guizhou University,Guiyang 550025,Guizhou,China;Institute of Plant Protection,Guizhou Academy of Agricultural Science,Guiyang 550006,Guizhou,China)

机构地区：[1]贵州大学生命科学学院,农业生物工程研究院,山地植物资源保护与种质创新教育部重点实验室,贵州贵阳550025 [2]贵州省农业科学院植物保护研究所,贵州贵阳550006

出　　处：《中国蔬菜》2024年第2期51-57,共7页China Vegetables

基　　金：贵州省农业科学院青年英才培育项目[黔农科院青年科技基金(2020)01号]。

摘　　要：用RT-PCR法对采自贵州6个地区的疑似辣椒病毒病样品进行了番茄斑萎病毒(TSWV)的鉴定,并用分子克隆方法扩增不同地区的感染TSWV的N基因序列。结果表明:贵州贵阳、安顺、瓮安、福泉、长顺和惠水的辣椒上均存在TSWV,经序列比对,贵州辣椒TSWV分离物核苷酸一致性为95.6%~99.7%,氨基酸一致性为97.1%~100.0%;通过系统进化树分析,福泉、惠水和长顺辣椒TSWV分离物与云南、山东的番茄TSWV分离物亲缘关系较近,安顺、贵阳和瓮安辣椒TSWV分离物与云南的番茄、辣椒、烟草、芹菜和鬼针草TSWV分离物,以及江苏的莴苣TSWV分离物亲缘关系较近。表明贵州辣椒TSWV分离物有2种株系,与国内不同地区不同寄主的TSWV分离物具有一定的相关性。Tomato spotted wilt virus(TSWV)was identified by RT-PCR in suspected pepper virus samples collected from 6 regions in Guizhou Province,and the N gene sequences of TSWV in different regions were amplified by molecular cloning.The results showed that TSWV were found in chilies from Guiyang,Anshun,Weng’an,Fuquan,Changshun and Huishui in Guizhou.After sequences aligning,the nucleotide and amino acid identities of the strains isolated from Guizhou were 95.6%-99.7%and 97.1%-100.0%,respectively.Phylogenetic tree analysis suggested that TSWV strains isolated from pepper in Fuquan,Huishui and Changshun were closely related to those in Yunnan and Shandong.The TSWV strains isolated from peppers in Anshun,Guiyang and Weng’an were relatively closed to the strains isolated from tomato,pepper,tobacco,celery and Herb of Spanish needles in Yunnan and lettuce in Jiangsu.The study indicated that there were two TSWV lines in Guizhou pepper,which were certainly correlated with those isolates from various hosts in different regions in China.

关 键 词：辣椒 番茄斑萎病毒 N基因 病毒鉴定 序列分析 

分 类 号：S436.418.1[农业科学—农业昆虫与害虫防治]