HPLC法测定广西不同产地八角茴香中4种有机酸的含量  

Determination of Four Organic Acids in Star Anise From Different Producing Areas in Guangxi by HPLC

作　　者：欧敏 潘宇 陈路[1,2] 赵成坚 侯小利[1,2] 谭栀恩 张孟丽 李晓玲 吴芳芳 OU Min;PAN Yu;CHEN Lu;ZHAO Cheng-jian;HOU Xiao-li;TAN Zhi-en;ZHANG Meng-li;LI Xiao-ling;WU Fang-fang(Medicinal Botnical Garden of Guangxi Zhuang Autonomous Region,Nanning,Guangxi,530023;National Engineering Laboratory for Development of Endangered Medicinal Resources in Southwest China,Nanning,Guangxi,530023;Youjiang Medical University for Nationalities,Baise,Guangxi,533000)

机构地区：[1]广西壮族自治区药用植物园,广西南宁530023 [2]西南濒危药材资源开发国家工程实验室,广西南宁530023 [3]右江民族医学院,广西百色533000

出　　处：《广西中医药大学学报》2024年第1期53-57,共5页Journal of Guangxi University Of Chinese Medicine

基　　金：国家自然科学基金项目(编号:82003932);中央引导地方科技发展资金项目(编号:桂科ZY21195044);广西自然科学基金项目(编号:2020JJA140044);广西壮族自治区中医药管理局自筹经费科研课题(编号:GXZYZ20210223);广西药用植物园科研基金项目(编号:桂药基201802);广西药用植物园科研基金项目(编号:桂药基2020010)。

摘　　要：[目的]建立八角茴香中莽草酸、原儿茶酸、对羟基苯甲酸、对甲氧基苯甲酸的含量测定方法。[方法]采用高效液相色谱法对八角茴香进行测定,色谱柱为Kromasil 100-5-C_(18)(250 mm×4.6 mm,5μm),流动相为乙腈-3%磷酸水溶液(梯度洗脱),流速为1.0 ml/min,进样量为10μl,检测波长为254 nm,柱温为40℃。[结果]广西11个不同产地的八角茴香中莽草酸含量为6.34%~7.41%,原儿茶酸含量为0.28%~0.98%,对羟基苯甲酸含量为0.73%~1.90%,对甲氧基苯甲酸含量为0.07%~0.23%。[结论]本研究建立的方法操作简单,稳定性强,重复性好,可用于八角茴香中有效成分的含量测定。[Objective]To establish a method for the determination of shikimic acid,protocatechuic acid,p-hy⁃droxybenzoic acid and p-methoxybenzoic acid in star anise.[Methods]The star anise was determined by high perfor⁃mance liquid chromatography.The chromatographic column was Kromasil 100-5-C_(18)(250 mm×4.6 mm,5μm).The mobile phase was acetonitrile-3%phosphoric acid aqueous solution(gradient elution).The flow rate was 1.0 ml/min,the injection volume was 10μl,the detection wavelength was 254 nm,and the column temperature was 40℃[.Results]The contents of shikimic acid,protocatechuic acid,p-hydroxybenzoic acid and p-methoxybenzoic acid in star anise from 11 different producing areas in Guangxi were 6.34%~7.41%,0.28%~0.98%,0.73%~1.90%and 0.07%~0.23%,respectively.[Conclusion]The method applied in this study is simple,stable and reproducible,and can be used for the determination of active ingredients in star anise.

关 键 词：八角茴香 莽草酸 原儿茶酸 对羟基苯甲酸 对甲氧基苯甲酸 高效液相色谱法 含量测定 

分 类 号：R284.1[医药卫生—中药学]