New perspectives on the genetic structure of dotted gizzard shad(Konosirus punctatus)based on RAD-seq  

作　　者：Ying Peng Yifan Liu Jiasheng Li Kun Zhang Xun Jin Sixu Zheng Yunpeng Wang Zhenming Lu Liqin Liu Li Gong Bingjian Liu 

机构地区：[1]National Engineering Laboratory of Marine Germplasm Resources Exploration and Utilization,Zhejiang Ocean University,Zhoushan 316022,China [2]National Engineering Research Center for Facilitated Marine Aquaculture,Marine Science and Technology College,Zhejiang Ocean University,Zhoushan 316022,China

出　　处：《Marine Life Science & Technology》2024年第1期50-67,共18页海洋生命科学与技术（英文）

基　　金：supported by the National Natural Science Foundation of China(NSFC)(No.41806156);Zhejiang Provincial Natural Science Foundation of China(LY22D060001&LY20C190008);Science and Technology Project of Zhoushan(2020C21016);Funds for Zhejiang Provincial Universities and Research Institutes(No.2021J008);Fund of Guangdong Provincial Key Laboratory of Fishery Ecology and Environment(FEEL-2021-8).

摘　　要：To maintain,develop and rationally utilize marine organisms,understanding their genetic structure and habitat adaptation pattern is necessary.Konosirus punctatus,which is a commercial fish species inhabiting the Indo-west Pacific Ocean,has shown an obvious annual global capture and aquaculture production decline due to climate changes and human activities.In the present study,restriction-site associated DNA sequencing(RAD-seq)was used to describe its genome-wide single nucleotide polymorphisms panel(SNPs).Among 146 individuals collected at nine locations scattered in China,Korea and Japan,a set of 632,090 SNPs were identified.Population genetic analysis showed that K.punctatus individuals were divided into two significant genetic clusters.Meanwhile,potential genetic differentiation between northern and southern population of K.punctatus was found.Treemix results indicated that gene flow existed among sampling locations of K.punctatus,especially from southern Japan to others.Moreover,candidate genes associated with habitat adaptations of K.punctatus were identified,which are involved in diverse physiological processes of K.punctatus including growth and development(e.g.,KIDINS220,PAN3),substance metabolism(e.g.,PGM5)and immune response(e.g.,VAV3,CCT7,HSPA12B).Our findings may aid in understanding the possible mechanisms for the population genetic structure and local adaptation of K.punctatus,which is beneficial to establish the management and conservation units of K.punctatus,guiding the rational use of resources,with reference significance for a profound understanding of the adaptative mechanisms of other marine organisms to the environment.

关 键 词：Population structure Konosirus punctatus SNP RAD-seq Local adaptation 

分 类 号：Q178.53[生物学—水生生物学]