Genome-resolved metagenomics of Venice Lagoon surface sediment bacteria reveals high biosynthetic potential and metabolic plasticity as successful strategies in an impacted environment  

作　　者：Elisa Banchi Erwan Corre Paola Del Negro Mauro Celussi Francesca Malfatti 

机构地区：[1]National Institute of Oceanography and Applied Geophysics OGS,Trieste,Italy [2]FR2424,Station Biologique de Roscoff,Plateforme ABiMS(Analysis and Bioinformatics for Marine Science),Sorbonne Universite CNRS,29680 Roscoff,France [3]Department of Life Sciences,University of Trieste,Trieste,Italy

出　　处：《Marine Life Science & Technology》2024年第1期126-142,共17页海洋生命科学与技术（英文）

摘　　要：Bacteria living in sediments play essential roles in marine ecosystems and deeper insights into the ecology and biogeochemistry of these largely unexplored organisms can be obtained from‘omics’approaches.Here,we characterized metagenome-assembled-genomes(MAGs)from the surface sediment microbes of the Venice Lagoon(northern Adriatic Sea)in distinct sub-basins exposed to various natural and anthropogenic pressures.MAGs were explored for biodiversity,major marine metabolic processes,anthropogenic activity-related functions,adaptations at the microscale,and biosynthetic gene clusters.Starting from 126 MAGs,a non-redundant dataset of 58 was compiled,the majority of which(35)belonged to(Alpha-and Gamma-)Proteobacteria.Within the broad microbial metabolic repertoire(including C,N,and S metabolisms)the potential to live without oxygen emerged as one of the most important features.Mixotrophy was also found as a successful lifestyle.Cluster analysis showed that different MAGs encoded the same metabolic patterns(e.g.,C fixation,sulfate oxidation)thus suggesting metabolic redundancy.Antibiotic and toxic compounds resistance genes were coupled,a condition that could promote the spreading of these genetic traits.MAGs showed a high biosynthetic potential related to antimicrobial and biotechnological classes and to organism defense and interactions as well as adaptive strategies for micronutrient uptake and cellular detoxification.Our results highlighted that bacteria living in an impacted environment,such as the surface sediments of the Venice Lagoon,may benefit from metabolic plasticity as well as from the synthesis of a wide array of secondary metabolites,promoting ecosystem resilience and stability toward environmental pressures.

关 键 词：Antibiotic resistance PAHs Biosynthetic gene clusters MICROSCALE Mobilome RESISTOME 

分 类 号：Q78[生物学—分子生物学]