淫羊藿提取物对老龄种公鸡精液品质、生殖内分泌激素及繁殖性能的影响  

Effects of Epimedium Extract on Semen Quality, Reproductive EndocrineHormone, Reproduction Performance of Aged Breeder Roosters

作　　者：韩书宇 孙海霞 刘静[1] 郭梦宇 代占辉 安胜英[1] 刘观忠[1] HAN Shuyu;SUN Haixia;LIU Jing;GUO Mengyu;DAI Zhanhui;AN Shengying;LIU Guanzhong(College of Animal Science and Technology,Hebei Agricultural University,Baoding 071000,China;Baoding Xingrui Agriculture and Animal Husbandry Development Co.,Ltd.,Baoding 071000,China)

机构地区：[1]河北农业大学动物科技学院,保定071000 [2]保定兴芮农牧发展有限公司,保定071000

出　　处：《动物营养学报》2024年第1期312-322,共11页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：河北省现代农业产体系蛋肉鸡创新团队建设专项;河北省科技特派员专项(HBCT2018502053)。

摘　　要：本试验旨在探究不同水平淫羊藿提取物对老龄种公鸡精液品质、生殖内分泌激素及繁殖性能的影响。选取72只体重[(2.94±0.01)kg]相近、健康的65周龄大午金凤父母代种公鸡,随机分成4组,每组6个重复,每个重复3只,单笼饲养。对照组饲喂基础饲粮,试验Ⅰ、Ⅱ、Ⅲ组分别在基础饲粮中添加0.05%、0.10%和0.15%的淫羊藿提取物。饲养试验持续7周。试验第4周(69周龄)和第7周(72周龄)收集精液,测定精液品质;试验第4周和第7周采集血液,测定血清生殖内分泌激素水平;试验最后2周收集合格种蛋以重复为单位入孵,用于测定繁殖性能指标。结果显示:与对照组相比,1)试验第4周时,各试验组种公鸡精子活力显著提高(P<0.05),精子畸形率显著降低(P<0.05);试验Ⅱ、Ⅲ组有效精子数显著增加(P<0.05);试验Ⅱ组精子密度显著提高(P<0.05)。试验第7周时,试验Ⅱ、Ⅲ组种公鸡精液量显著增加(P<0.05);试验Ⅲ组精子活力显著提高(P<0.05)。2)试验第4周时,试验Ⅱ、Ⅲ组精浆谷草转氨酶(GOT)、谷丙转氨酶(GPT)活性显著降低(P<0.05);试验第7周时,试验Ⅲ组精浆GOT、GPT活性显著降低(P<0.05)。3)试验第4周时,各试验组精浆谷胱甘肽过氧化物酶(GSH-Px)活性极显著提高(P<0.01);试验Ⅱ、Ⅲ组精浆总抗氧化能力(T-AOC)显著提高(P<0.05)。试验第7周时,各试验组精浆总超氧化物歧化酶(T-SOD)活性显著提高(P<0.05);试验Ⅱ、Ⅲ组精浆丙二醛(MDA)含量显著降低(P<0.05);试验Ⅱ组精浆T-AOC显著提高(P<0.05)。4)试验第4周时,各试验组血清促性腺激素释放激素(GnRH)水平显著降低(P<0.05),血清促黄体素(LH)和睾酮(T)水平显著提高(P<0.05);试验Ⅱ、Ⅲ组血清促卵泡素(FSH)水平显著提高(P<0.05)。试验第7周时,各试验组血清GnRH水平极显著降低(P<0.01);试验Ⅱ、Ⅲ组血清FSH、LH水平显著提高(P<0.05);试验Ⅲ组血清T水平显著提高(P<0.05)。5)试验Ⅱ、Ⅲ组受精率和�The experiment was to investigate the effects of different levels of epimedium extract on semen quality,reproductive endocrine hormone,reproduction performance of aged breeder roosters.Seventy-two healthy 65-week-old Dawu Jinfeng parent breeder roosters with similar body weight[(2.94±0.01)g]were randomly divided into 4 groups with 6 replicates per group and 3 breeder roosters per replicate,and the roosters fed separately.Breeder roosters in the control group were fed a basal diet,and breeder roosters in trial groupsⅠ,ⅡandⅢwere fed diets supplemented with 0.05%,0.10%and 0.15%epimedium extract,respectively.The feeding experiment lasted for 7 weeks.At the 4th(69-week-old)and 7th week(72-week-old)of experiment,semen was collected to measure semen quality,and blood was collected to measure reproductive endocrine hormone levels;qualified breeding eggs were collected and incubated them on a repeated basis at last two weeks of experiment to measure reproduction performance indexes.The results showed that,compared with the control group,1)at the 4th week of the experiment,the sperm motility was significantly increased in all trial groups(P<0.05),the sperm abnormality was significantly decreased in trial groups(P<0.05),the number of available sperm in trial groupsⅡandⅢwas significantly increased(P<0.05),and the sperm density in trial groupⅡwas significantly increased(P<0.05).At the 7th week of experiment,the ejaculation volume of trial groupsⅡandⅢwas significantly increased(P<0.05),and the sperm motility was significantly increased in trial groupⅢwas significantly increased(P<0.05).2)At the 4th week of the experiment,the seminal plasma glutamic oxalacetic transaminase(GOT)and glutamic-pyruvic transaminase(GPT)activities in trial groupsⅡandⅢwere significantly decreased(P<0.05);at the 7th week of the experiment,the seminal plasma GOT and GPT activities in trial groupⅢwas significantly decreased(P<0.05).3)At the 4th week of the experiment,the glutathione peroxidase(GSH-Px)activity was in all trial gro

关 键 词：淫羊藿提取物 老龄种公鸡 精液品质 生殖内分泌激素 繁殖性能 

分 类 号：S816[农业科学—饲料科学]