N-酰基高丝氨酸内酯酶在肉鸡中应用的耐受性评价  

Evaluation of Tolerance of N-Acyl-Homoserine Lactonase in Broiler Chickens

作　　者：陈星 郑爱娟[1] 陈志敏[1] 张广民[1] 王泽栋 刘国华[1] 常文环[1] 蔡辉益[1] CHEN Xing;ZHENG Aijuan;CHEN Zhimin;ZHANG Guangmin;WANG Zedong;LIU Guohua;CHANG Wenhuan;CAI Huiyi(Key Laboratory of Feed Biotechnology of the Ministry of Agriculture and Rural Affairs,Risk Assessment Laboratory of Animal Product Quality Safety Feed Source Factors of the Ministry of Agriculture and Tural Affairs,Feed Research Institute,Chinese Academy of Agricultural Sciences,Beijing 100081,China)

机构地区：[1]中国农业科学院饲料研究所,农业农村部动物产品质量安全饲料源性因子风险评估实验室,农业农村部饲料生物技术重点实验室,北京100081

出　　处：《动物营养学报》2024年第2期885-896,共12页CHINESE JOURNAL OF ANIMAL NUTRITION

基　　金：国家重点研发计划项目(2023YFD1301602);国家肉鸡产业技术体系(CARS-41)。

摘　　要：本试验依据《饲料和饲料添加剂畜禽靶动物耐受性评价试验指南(试行)》对N-酰基高丝氨酸内酯酶(AHLase)在肉鸡中的应用进行耐受性评价。采用双因素试验设计,选取健康、体重接近的1日龄科宝肉仔鸡360只,随机分为3组,每组6个重复,每个重复20只鸡,公母各占1/2且分笼饲养。对照组饲喂基础饲粮,试验组在基础饲粮中分别添加500(AHLase-500)和5000 mg/kg(AHLase-5000)的AHLase。试验期42 d。结果表明:1)与对照组相比,AHLase-5000组公鸡1~21日龄的平均日采食量显著提高(P<0.05),22~42日龄以及1~42日龄的料重比显著降低(P<0.05);AHLase-5000组母鸡22~42日龄末重显著提高(P<0.05),而AHLase-500、AHLase-5000组1~42日龄母鸡的平均日采食量显著降低(P<0.05)。2)试验期内公鸡的平均日增重、平均日采食量显著高于母鸡(P<0.05)。1~21日龄公鸡的料重比有高于母鸡的趋势(P=0.075)。性别对1~21日龄肉鸡末重表现出趋于显著的影响(P=0.079)。3)随着AHLase添加水平的增加,1~21日龄肉鸡的平均日采食量显著提高(P<0.05),平均日增重有提高的趋势(P=0.052),而料重比显著降低(P<0.05);22~42日龄肉鸡的末重显著提高(P<0.05)。4)AHLase添加水平和性别在1~21日龄平均日采食量、22~42日龄平均日采食量以及1~42日龄料重比中均表现出显著互作效应(P<0.05);在22~42日龄料重比(P=0.069)以及1~42日龄平均日增重(P=0.064)中表现出互作趋势。5)饲粮中添加AHLase对肉鸡血常规和血清生化指标无显著影响(P>0.05)。6)饲粮中添加AHLase对肉鸡器官指数无显著影响(P>0.05),组织未观察到形态结构变化。由此可见,饲粮中添加5000 mg/kg的AHLase改善了肉鸡生长性能,对血液生理生化指标和内脏器官发育无显著影响,表明肉鸡对AHLase的耐受剂量在5000 mg/kg以上。This study aimed to evaluate the tolerance of N-acyl-homoserine lactonase(AHLase)in broilers application,following the Guidelines for Evaluation of Tolerance of Livestock and Poultry Target Animals in Feed and Feed Additives(Trial).A total of 360 one-day-old Kebao broiler chickens,with similar health and weight,were randomly divided into 3 groups.Each group had 6 replicates,with 20 chickens in each replicate.The replicates consisted of 1/2 cocks and 1/2 hens,and the chickens were raised in separate cages.The control group was fed a basic diet,while the experimental groups were supplemented with 500(AHLase-500)and 5000 mg/kg(AHLase-5000)of AHLase in the basic diet,respectively.The experimental period lasted for 42 days.The results showed as follows:1)the average daily feed intake of cocks aged 1 to 21 days in the AHLase-5000 group significantly increased compared with the control group(P<0.05);the feed to gain of cocks aged 22 to 42 days and 1 to 42 days significantly decreased(P<0.05).The final weight of hens aged 22 to 42 days in the AHLase-5000 group significantly increased(P<0.05),while the average daily feed intake of hens aged 1 to 42 days in the AHLase group significantly decreased(P<0.05).2)Throughout the experimental period,cocks exhibited significantly higher average daily gain and average daily feed intake compared with hens(P<0.05).The feed to gain of cocks aged 1 to 21 days showed a trend higher than that of hens(P=0.075).Gender showed a significant trend in the final weight of broiler chickens aged 1 to 21 days(P=0.079).3)The supplementation of AHLase led to a significant increase in the average daily feed intake of broilers aged 1 to 21 days(P<0.05).There was also an increasing trend in the average daily gain(P=0.052),and a significant decrease in the feed to gain(P<0.05).The final weight of broiler chickens aged 22 to 42 days significantly increased(P<0.05).4)The interaction effects of AHLase supplementation level and gender were observed in the average daily feed intake from 1 to 21 days of age,av

关 键 词：N-酰基高丝氨酸内酯酶 肉鸡 生长性能 器官指数 耐受性 

分 类 号：S831[农业科学—畜牧学]