功能性电刺激改善脊髓损伤后肌肉萎缩的生物信息学分析  

Comprehensive bioinformatic analysis of functional electrical stimulation ameliorating muscular atrophy after spinal cord injury

作　　者：王莉华 臧春雁 闵军[1] 范筱 戴世友[3] Wang Lihua;Zang Chunyan;Min Jun;Fan Xiao;Dai Shiyou(Department ofEmergency,Fuzhou Second Hospital,Fuzhou,Fujian 350007,China;Department ofEmergency,Western District of Qingdao Municipal Hospital,Qingdao,Shandong 266071,China;Department of Bone and Joint and Sports Medicine,Eastern District of QingdaoMunicipal Hospital,Qingdao,Shandong 266071,China)

机构地区：[1]福州市第二医院急诊科,福建福州350007 [2]青岛市市立医院西院区急诊科,山东青岛266071 [3]青岛市市立医院东院区关节与运动医学科,山东青岛266071

出　　处：《创伤与急诊电子杂志》2023年第4期162-170,共9页Journal of Trauma and Emergency(Electronic Version)

基　　金：山东省中医药科技发展计划(2019-0614);福建省第一批临床医学研究中心(2020Y2014)。

摘　　要：目的利用生物信息学方法探究功能性电刺激促进脊髓损伤后肌肉萎缩患者肌肉功能恢复的生物学调控因子及其作用机制。方法从美国国立生物技术信息中心基因表达数据库下载相关数据集GSE142426和GSE33886。利用R语言整合数据集、校正批间差、筛选差异基因。利用在线工具DAVID进行基因本体论及京都基因和基因组百科全书通路富集,使用STRING数据库进行差异编码蛋白的相互作用分析。选取关键基因绘制受试者操作特征曲线,选取最佳预测基因进行基因集富集分析。结果共筛选出114个差异基因,包含44个高表达基因和70个低表达基因,其中CYCS、SUCLG1、ATP5B、ATP5C1被认为是4个关键基因。受试者操作特征曲线提示CYCS是该研究的最佳预测基因,基因组富集分析提示CYCS的上调主要与线粒体能量代谢相关。结论功能性电刺激促进脊髓损伤后肌肉萎缩患者的肌肉功能恢复,可能是通过调控CYCS基因影响线粒体能量代谢实现的。Objective To explore the biological regulatory factors and their mechanisms related to functional electrical stimulation for muscle function recovery of patients with muscular atrophy after spinal cord injury using bioinformatics methods.Method The related microarray datasets GSE142426 and GSE33886 were downloaded from the Gene Expression Omnibus database.We used the R software to merge the two datasets,correct the inter-batch differences and screen the differentially expressed genes.Gene Ontology enrichment analysis and Kyoto Encyclopedia of Genes and Genomes pathway were analyzed by using the DAVID online tool.The STRING database was used to analyze the interaction of differentially encoded proteins.The key genes were selected to draw the receiver operating characteristic curve,and CYCS was used to perform gene set enrichment analysis.Result A total of 114 differentially expressed genes were selected,including 44 up-regulated genes and 70 down-regulated genes,and four hub genes were identified including CYCS,SUCLG1,ATP5B and ATP5C1.Receiver operating characteristic curve showed CYCS to be the best indicator,and gene set enrichment analysis showed that up-regulation of CYCS was associate with mitochondria and energy metabolism.Conclusion The mechanism of functional electrical stimulation in muscle recovery for spinal cord injury patients with muscular atrophy is potentially involved in regulating mitochondrial energy metabolism by CYCS.

关 键 词：脊髓损伤 功能性电刺激 肌肉萎缩 生物信息学 

分 类 号：R651.2[医药卫生—外科学]