拟杆菌属临床分离株耐药性及脆弱拟杆菌肠毒素bft基因分型特征分析  

Drug resistance of Bacteroides isolated from clinic and characteristics of Bacteroides fragilis bft genotyping

作　　者：王艳艳[1] 王俊瑞[1] 郑文琪[1] 兰海霞 郭素芳[1] WANG Yanyan;WANG Junrui;ZHENG Wenqi;LAN Haixia;GUO Sufang(Department of Clinical Laboratory,the Affiliated Hospital of Inner Mongolia Medical University,Hohhot 100050,Inner Mongolia,China;Department of Pediatrics,the 969th Hospital of Chinese People's Liberation Army,Hohhot 100051,Inner Mongolia,China)

机构地区：[1]内蒙古医科大学附属医院检验科,内蒙古呼和浩特100050 [2]解放军第九六九医院儿科,内蒙古呼和浩特100051

出　　处：《检验医学》2024年第1期47-52,共6页Laboratory Medicine

基　　金：内蒙古自治区自然科学基金面上项目(2021MS08156);内蒙古自治区高等学校研究自然科学一般项目(NJZY21627)。

摘　　要：目的 分析拟杆菌属临床分布及其耐药特征,并探讨脆弱拟杆菌肠毒素bft基因分型特征。方法收集2016年6月—2019年6月内蒙古医科大学附属医院分离自临床样本的拟杆菌属147株,对其进行菌种鉴定和体外药物敏感性试验。采用聚合酶链反应(PCR)检测脆弱拟杆菌肠毒素bft基因。结果 147株拟杆菌属细菌中,脆弱拟杆菌100株(68.0%)、多形拟杆菌25株(17.0%)、卵形拟杆菌9株(6.1%)。体外药物敏感性试验结果显示,拟杆菌属细菌对克林霉素的耐药率最高(78.9%);脆弱拟杆菌对克林霉素的耐药率为85.0%,高于其他拟杆菌(66.0%)。拟杆菌属细菌对亚胺培南和甲硝唑的耐药率分别为13.6%和4.7%,对其他抗菌药物呈不同程度耐药;脆弱拟杆菌对大部分抗菌药物的耐药率均高于其他类型拟杆菌。脆弱拟杆菌bft基因阳性率为38.0%,以bft-1亚型为主要基因型(28.0%),其次为bft-2亚型(10.0%),未检出bft-3亚型。分离自非腹部临床样本的脆弱拟杆菌bft基因阳性率(55.6%)高于分离自腹部临床样本的脆弱拟杆菌(36.3%)。结论 拟杆菌属临床分离株中脆弱拟杆菌分离率最高,其对临床常用抗菌药物均有一定的耐药性。分离自非腹部临床样本的脆弱拟杆菌产肠毒素的菌株流行率更高,应引起临床重视。Objective To investigate the distribution and drug resistance characteristics of Bacteroides,and to investigate the genotyping characteristics of bft gene in Bacteroides fragilis.Methods Totally,147 isolates of Bacteroides were collected from the Affiliated Hospital of Inner Mongolia Medical University from June 2016 to June 2019,and they were identified by in vitro drug susceptibility test.Polymerase chain reaction(PCR)was used to determine bft gene in Bacteroides fragilis.Results In the 147 isolates of Bacteroides,100 isolates of Bacteroides fragilis were determined,accounting for 68.0%,25 isolates of Bacteroides thetaiotaomicron accounted for 17.0%,and 9 isolates of Bacteroides ovalis accounted for 6.1%.The highest drug resistance rate of Bacteroides was 78.9%to clindamycin.The clindamycin resistance rate for Bacteroides fragilis was 85.0%,which was higher than those of other Bacteroides(66.0%).Imipenem and meropenem resistance rates were 13.6%and 4.7%for Bacteroides,respectively.Bacteroides showed different degrees of resistance to other drugs,and the most drug resistance rates of Bacteroides fragilis to drugs were higher than those of other Bacteroides.The positive rate of bft gene was 38.0%in Bacteroides fragilis.The main genotype was bft-1(28.0%),followed by bft-2(10.0%),and no bft-3 was found.The positive rate of bft gene of Bacteroides fragilis isolated from non-abdominal specimens(55.6%)was higher than that of Bacteroides fragilis isolated from abdominal specimens(36.3%).Conclusions Bacteroides fragilis has the highest isolation rate in Bacteroides,and it has certain resistance to common clinical drugs.The prevalence of enterotoxin-producing isolates of Bacteroides fragilis isolated from non-abdominal specimens is higher,which should be widely paid attention in clinic.

关 键 词：拟杆菌属 脆弱拟杆菌 耐药性 bft基因 

分 类 号：R378.99[医药卫生—病原生物学]