CD4^(+)T细胞亚型及其相关炎性因子在NE-FE-COPD患者中的表达及意义  

Expression and significance of CD4^(+)T cell subtypes and their associated inflammatory factors in NE-FE-COPD patients

作　　者：杨春晓 叶婷[2] 余维巍 YANG Chunxiao;YE Ting;YU Weiwei(Department of Geriatric Medicine,Tongji Hospital Affiliated to Tongji Medical College of Huazhong University of Science and Technology,Wuhan,Hubei 430030,China;Department of Clinical Nutrition,Tongji Hospital Affiliated to Tongji Medical College of Huazhong University of Science and Technology,Wuhan,Hubei 430030,China)

机构地区：[1]华中科技大学同济医学院附属同济医院老年医学科,武汉430030 [2]华中科技大学同济医学院附属同济医院临床营养科,武汉430030

出　　处：《重庆医学》2024年第4期502-507,共6页Chongqing medicine

基　　金：国家自然科学基金青年基金项目(81500032)。

摘　　要：目的探讨中性粒细胞优势型频繁急性加重型的慢性阻塞性肺疾病(NE-FE-COPD)患者CD4^(+)T细胞亚型及其相关炎性因子的表达及意义。方法选取2019年3月至2021年3月在该院治疗的COPD患者为研究对象,按照不同表型分为非频繁急性加重型COPD组(IE-COPD组,n=11)、嗜酸性粒细胞优势型频繁急性加重型COPD组(Eos-FE-COPD组,n=13)、中性粒细胞优势型频繁急性加重型COPD组(NE-FE-COPD组,n=15),肺功能正常及吸烟史>10包/年者为对照组(CTRL组,n=9)。采集各组支气管肺泡灌洗液(BALF),流式细胞术检测CD4^(+)T细胞亚型表达,ELISA测定炎性因子水平,分析其与肺功能和急性加重频率的相关性。CD4^(+)CD28^(null)T细胞和CD4^(+)CD28^(+)T细胞与人气道上皮细胞(hAECs)进行共培养后分为co-culture组和Control组,免疫荧光染色观察hAECs紧密连接损伤情况,RT-qPCR和Western blot检测ZO-1和occludin mRNA及蛋白表达水平。结果NE-FE-COPD组BALF中CD4^(+)CD28^(null)T细胞占比、白细胞介素(IL)-1β水平高于CTRL组、IE-COPD组、Eos-FE-COPD组,差异有统计学意义(P<0.05)。CD4^(+)CD28^(null)T细胞占比、IL-1β水平与肺功能呈负相关(P<0.05),与急性加重频率呈正相关(P<0.05)。与Control组比较,co-culture组hAECs紧密连接受损,ZO-1和occludin mRNA及蛋白表达水平降低,差异有统计学意义(P<0.05)。结论CD4^(+)CD28^(null)T细胞和IL-1β可能参与NE-FE-COPD的发生和发展。Objective To investigate the expression of CD4^(+)T cell subtypes and related inflammatory factors in patients with neutrophil-predominant frequent acute exacerbation chronic obstructive pulmonary disease(NE-FE-COPD).Methods COPD patients who were treated in the hospital from March 2019 to March 2021 were selected as the research objects.According to different phenotypes,they were divided into the infrequent exacerbator COPD group(IE-COPD group,n=11),the eosinophilic dominant frequent acute plus severe COPD group(Eos-FE-COPD group,n=13),and the neutrophil dominant frequent exacerbator COPD group(NE-FE-COPD group,n=15).Patients with normal lung function and smoking history>10 packs/year were the control group(CTRL group,n=9).Bronchoalveolar lavage fluid(BALF)was collected from each group,and the expression of CD4^(+)T cell subtypes and inflammatory factors were detected by flow cytometry.The correlation between BALF and lung function and the frequency of acute exacerbation was analyzed.CD4^(+)CD28^(null) T cells and CD4^(+)CD28^(+)T cells were co-cultured with human airway epithelial cells(hAECs)and divided into co-culture group and Control group.The damage of hAECs was observed by immunofluorescence staining,and the mRNA and protein expression levels of ZO-1 and occludin were detected by RT-qPCR and Western blot.Results The proportion of CD4^(+)CD28^(null) T cells and IL-1βlevel in BALF in the NE-FE-COPD group were higher than those in the CTRL group,the IE-COPD group,and the Eos-FE-COPD group,and the difference was statistically significant(P<0.05).The proportion of CD4^(+)CD28^(null) T cells and IL-1βlevel were negatively correlated with lung function(P<0.05),and positively correlated with acute exacerbation frequency(P<0.05).Compared with the Control group,hAECs tight junctions were damaged in the co-culture group,and mRNA and protein expression levels of ZO-1 and occludin decreased,with statistical significance(P<0.05).Conclusion CD4^(+)CD28^(null) T cells and IL-1βmay be involved in the occurrence and dev

关 键 词：慢性阻塞性肺疾病 CD4^(+)CD28^(null)T细胞 白细胞介素-1Β 

分 类 号：R563.3[医药卫生—呼吸系统]