PatU3 plays a central role in coordinating cell division and differentiation in pattern formation of filamentous cyanobacterium Nostoc sp.PCC 7120  

作　　者：Lei Yin Zhenggao Zheng Yilin Li Xiying Li Dan Cheng Chunxia Dong Yixuan Liu Jindong Zhao 

机构地区：[1]State Key Laboratory of Protein and Plant Genetic Engineering,School of Life Sciences,Peking University,Beijing 100871,China [2]National Teaching Center for Experimental Biology,School of Life Sciences,Peking University,Beijing 100871,China [3]Institute of Hydrobiology,Chinese Academy of Sciences,Wuhan 430072,China

出　　处：《Science China(Life Sciences)》2023年第12期2896-2909,共14页中国科学（生命科学英文版）

基　　金：supported by the National Natural Science Foundation of China (32070203);the National Key Research and Development Program of China (2017YFA0503703),National Key Research and Development Program of China (2019YFA0904700,2021YFA0910700,2021YFA0909700);Qidong-SLS Innovation Fund (202001539)。

摘　　要：Spatial periodic signal for cell differentiation in some multicellular organisms is generated according to Turing's principle for pattern formation.How a dividing cell responds to the signal of differentiation is addressed with the filamentous cyanobacterium Nostoc sp.PCC 7120,which forms the patterned distribution of heterocysts.We show that differentiation of a dividing cell was delayed until its division was completed and only one daughter cell became heterocyst.A mutant of patU3,which encodes an inhibitor of heterocyst formation,showed no such delay and formed heterocyst pairs from the daughter cells of cell division or dumbbell-shaped heterocysts from the cells undergoing cytokinesis.The patA mutant,which forms heterocysts only at the filament ends,restored intercalary heterocysts by a single nucleotide mutation of patU3,and double mutants of patU3/patA and patU3/hetF had the phenotypes of the patU3 mutant.We provide evidence that HetF,which can degrade PatU3,is recruited to cell divisome through its C-terminal domain.A HetF mutant with its N-terminal peptidase domain but lacking the C-terminal domain could not prevent the formation of heterocyst pairs,suggesting that the divisome recruitment of HetF is needed to sequester HetF for the delay of differentiation in dividing cells.Our study demonstrates that PatU3 plays a key role in celldivision coupled control of differentiation.

关 键 词：CYANOBACTERIA heterocyst differentiation cell division pattern formation PatU3 

分 类 号：Q932[生物学—微生物学]