Hydrangea serrata extract exerts tumor inhibitory activity against hepatocellular carcinoma HepG2 cells via inducing p27/CDK2-mediated cell cycle arrest and apoptosis  

作　　者：Ye-eun Kim Jeonghye Hwang Ki-Young Kim 

机构地区：[1]Graduate School of Biotechnology,Kyung Hee University,Seocheon,Giheung,Yongin,Gyeonggi-do 446-701,Republic of Korea [2]Department of Genetics and Biotechnology,College of Life Sciences,Kyung Hee University,Seocheon,Giheung,Yongin,Gyeonggi-do 446-701,Republic of Korea

出　　处：《Asian Pacific Journal of Tropical Biomedicine》2024年第2期65-72,I0002-I0005,共12页亚太热带生物医学杂志（英文版）

基　　金：funded by the GRRC Program of Gyeonggi province[GRRC-KyungHee2023(B01)],Republic of Korea.

摘　　要：Objective:To examine the inhibitory effect of Hydrangea serrata extract against hepatocellular carcinoma HepG2 cells and its underlying mechanisms.Methods:The effects of Hydrangea serrata extract on growth inhibition of tumor cells and spheroids were assessed using MTT and 3D culture assays.Quantitative real-time PCR and Western blot analyses were employed to investigate the changes in mRNA and protein expression levels of molecules related to cell cycle and apoptosis.Results:Hydrangea serrata extract effectively inhibited the growth of both tumor cells and spheroids.The extract also significantly upregulated p27 mRNA expression and downregulated CDK2 mRNA expression,leading to cell cycle arrest.Moreover,increased BAX/Bcl-2 ratio as well as caspase-9 and-3 were observed after treatment with Hydrangea serrata extract,indicating the induction of tumor cell apoptosis.Conclusions:Hydrangea serrata extract has the potential to alleviate tumors by effectively modulating cell-cycle-related gene expressions and inducing apoptosis,thereby inhibiting tumor growth.

关 键 词：Hydrangea serrata Hepatocellular carcinoma Liver cancer Anticancer Cell cycle arrest APOPTOSIS 

分 类 号：R285.1[医药卫生—中药学]