维吾尔族2型糖尿病患者外周血中环状RNA生物标志物的研究  

Study on the biomarker of circular RNA in peripheral blood of Uygur patients with type 2 diabetes mellitus

作　　者：朱余蓉[1] 巴涛 康洁红 孙媛 常向云[1] ZHU Yurong;BA Tao;KANG Jiehong(Department of Endocrinology and Metabolism,First Affiliated Hospital,Shihezi University,Shihezi 832008,China)

机构地区：[1]石河子大学第一附属医院内分泌代谢科,832008

出　　处：《中国糖尿病杂志》2024年第1期6-10,共5页Chinese Journal of Diabetes

基　　金：国家自然科学基金(81860149);石河子大学科研基金项目(ZZZC202073A)。

摘　　要：目的探讨维吾尔族T2DM患者潜在的环状RNA(circRNA)生物标志物。方法选取2020年10月至2021年8月于石河子大学第一附属医院内分泌代谢科门诊及石河子社区招募的T2DM患者和糖耐量正常人群,其中维吾尔族T2DM患者60例(维吾尔族T2DM组)和维吾尔族糖耐量正常者60名(维吾尔族NC组),汉族T2DM患者60例(汉族T2DM组)和汉族糖耐量正常者60名(汉族NC组)。选取hsa_circRNA_0042817、hsa_circRNA_0006532、hsa_circRNA_0004131为候选circRNA,采用RT-qPCR检测其在外周血中表达。Logistic回归分析维吾尔族T2DM的影响因素,受试者工作特征(ROC)曲线分析circRNA在维吾尔族T2DM患者中的诊断价值。结果维吾尔族T2DM组hsa_circRNA_0042817、hsa_circRNA_0006532和hsa_circRNA_0004131表达高于维吾尔族NC组(P<0.05),hsa_circRNA_0042817表达高于汉族T2DM组(P<0.05)。Logistic回归分析显示,hsa_circRNA_0042817是维吾尔族T2DM的影响因素(OR 3.42095%CI 1.567~7.465)。ROC曲线分析显示,hsa_circRNA_0042817曲线下面积最大为0.798。结论维吾尔族T2DM患者外周血中存在表达上调的circRNA,hsa_circRNA_0042817可能是维吾尔族T2DM患者的生物标志物。Objective To explore the potential circular RNA(circRNA)biomarker of Uygur type 2diabetes mellitus(T2DM).Methods A total of 120 T2DM patients and 120 subjects with normal glucose tolerance were recruited from the Department of Endocrinology and Metabolism of the First Affiliated Hospital of Shihezi University and Shihezi community from October 2020 to August 2021,and divided into four groups:60 Uygur T2DM patients(Uygur T2DM group),60 Uygur subjects with normal glucose tolerance(Uygur NC group),60 Han T2DM patients(Han T2DM group)and 60 Han subjects with normal glucose tolerance(Han NC group).Hsa_circRNA_0042817,hsa_circRNA_0006532 and hsa_circRNA_0004131were selected as candidate circRNA,and the expression in peripheral blood were detected by RT-qPCR.Logistic regression was used to analyze the influencing factors for Uygur T2DM,and the receiver operating characteristic(ROC)curve was used to evaluate the biomarker value of circRNA in Uygur T2DM.Results The expressions of hsa_circRNA_0042817,hsa_circRNA_0006532 and hsa_circRNA_0004131were higher in Uygur T2DM group than in Uygur NC group(P<0.05).The expression of hsa_circRNA_0042817 was higher in Uygur T2DM group than in Han T2DM group(P<0.05).Logistic regression analysis showed that hsa_circRNA_0042817 was an influencing factor for T2DM in Uygur population[OR(95%CI)3.420(1.567~7.465)].ROC curve analysis showed that the area under the curve was the largest(0.798)in hsa_circRNA_0042817.Conclusion There were up-regulated circRNA in peripheral blood in Uygur T2DM patients,and hsa_circRNA_0042817 may be a biomarker for T2DM in Uygur patients.

关 键 词：糖尿病 2型 生物标志物 环状RNA 维吾尔族 

分 类 号：R587.1[医药卫生—内分泌]