2型糖尿病患者内脂素基因位点rs61330082、rs4730153多态性的研究  

Relationship between polymorphism of Visfatin gene rs61330082,rs4730153 and type 2 diabetes mellitus

作　　者：吐尼沙古丽·扎克尔 刘芬[1] 罗俊一[1] 赵倩[1] 杜国利[2] 张雪鹤 李晓梅[1] 杨毅宁[1] TUNISHAGUL·Zhaker;LIU Fen;LUO Junyi(Department of Coronary Heart Disease,The First Affiliated Hospital of Xinjiang Medical University,Urumqi 830054,China)

机构地区：[1]新疆医科大学第一附属医院心脏中心冠心病一科,乌鲁木齐830054 [2]新疆医科大学第一附属医院内分泌科,乌鲁木齐830054

出　　处：《中国糖尿病杂志》2024年第1期11-15,共5页Chinese Journal of Diabetes

基　　金：新疆维吾尔自治区重点研发计划项目(2020B03002、2020B03002-1、2020B03002-2、2020B03002-3)。

摘　　要：目的探讨内脂素基因位点rs61330082、rs4730153多态性与T2DM的相关性。方法利用SNPscanTM高通量单核苷酸多态性分型技术,对346例确诊T2DM患者(T2DM组)及1426名非T2DM对照人群(NC组)的内脂素基因位点rs61330082、rs4730153进行基因分型。Logistic回归分析T2DM的影响因素,受试者工作特征(ROC)曲线分析内脂素基因位点rs61330082、rs4730153对T2DM的诊断价值。结果T2DM组女性比例、年龄、肥胖、吸烟史、高血压、FPG、HbA1c、TG高于NC组(P<0.01),HDL-C低于NC组(P<0.01)。与NC组比较,T2DM组G等位基因和GG基因型频率升高(P<0.05)。Logistic回归分析显示,年龄、女性、肥胖、高血压、TG、rs4730153位点GG基因型是T2DM的危险因素,HDL-C是T2DM的保护因素。ROC曲线分析显示,曲线下面积为0.668,最佳切点值为20.04%,敏感度60.1%,特异度66.1%。结论内脂素基因位点rs4730153突变的GG基因型与T2DM风险相关,可作为预测T2DM表型变化的候选基因。Objective To evaluate the value of rs61330082 and rs4730153 polymorphisms of Visfatin locus for the diagnosis of type 2 diabetes mellitus(T2DM)in a high-risk population.Methods SNPscanTMhigh-throughput single nucleotide polymorphism typing technique was used to genotype Visfatin gene loci rs61330082 and rs4730153 in 346 T2DM patients(T2DM group)and 1426 normal controls(NC group).Logistic regression analysis was used to analyze T2DM risk factors.ROC curves were used to analyze the optimal cut-off values of Visfatin gene rs61330082 and rs4730153 for the diagnosis of T2DM.ResultsThe proportion of women,age,obesity,smoking,hypertension,FPG,HbA_1c and TG were higher in T2DM group than those in NC group(P<0.01)and HDL-C was lower than in NC group(P<0.01).The frequency of G allele and GG genotype was higher in T2DM group compared with NC group(P<0.05).Logistic regression analysis showed that age,female,obesity,hypertension,TG,and GG genotype at rs4730153 locus were risk factors for T2DM,HDL-C was a protective factor for T2DM.The area under the ROC curve of GG genotype at Visfatin rs4730153 mutation for diagnosis of T2DM was0.668 and the optimal cut-off point for predicting T2DM was 20.04%,with sensitivity 60.1%and specificity66.1%,respectively.Conclusion The GG genotype of Visfatin gene rs4730153 locus is associated with the risk of T2DM and can beused as a candidate gene for predicting phenotype of T2DM.

关 键 词：糖尿病 2型 内脂素 基因多态性 

分 类 号：R587.1[医药卫生—内分泌]