Expression and Characterization of a Novelλ-Carrageenase Cgl150A_Wa from Wenyingzhuangia aestuarii  

作　　者：SUN Yuhao CAO Siqi ZHANG Yuying XUE Changhu XIAO Hang CHANG Yaoguang 

机构地区：[1]College of Food Science and Engineering,Ocean University of China,Qingdao 266003,China [2]Marine Life Research Center,Laoshan National Laboratory,Qingdao 266237,China [3]Department of Food Science,University of Massachusetts Amherst,MA 01003,United States

出　　处：《Journal of Ocean University of China》2024年第1期209-215,共7页中国海洋大学学报（英文版）

基　　金：supported by the Fundamental Research Funds for the Central Universities(No.202012020);the National Key R&D Program of China(No.2018YFC 0311203).

摘　　要：λ-Carrageenan is a highly sulfated polysaccharide alternating of 1,4-O-α-D-galactopyranose-2,6-sulfate(D2S,6S)and 1,3-O-β-D-galactopyranose-2-sulfate(G2S).λ-Carrageenases are desirable tools forλ-carrageenan degradation.Based on the genome mining,a novelλ-carrageenase Cgl150A_Wa was cloned from the bacterium Wenyingzhuangia aestuarii and expressed in Escherichia coli.Cgl150A_Wa was an endo-acting enzyme and exhibited its maximum activity at 30℃and pH 8.0.By employing a glycomics strategy that combined ultra-performance liquid chromatography-mass spectrometry analysis and glycoinformatics,Cgl150A_Wa was proven to degradeλ-carrageenan octaose and hexaose,and the major hydrolysis product of Cgl150A_Wa wasλ-carrageenan tetrose.In addition to the typicalλ-carrageenan motifs,the active center of Cgl150A_Wa might tolerate desulfatedλ-carrageenan motifs.Cgl150A_Wa is a potential biotechnological tool for preparingλ-carrageenan oligosaccharides and structural investigation.

关 键 词：CARRAGEENAN λ-carrageenase LC-MS OLIGOSACCHARIDE GH150 

分 类 号：Q789[生物学—分子生物学]