LIF调控奶牛子宫内膜上皮细胞容受性基因表达研究  

LIF regulates the expression of receptive genes in bovine endometrial epithelial cells

作　　者：王红战 陈艳茹 李琦 唐颖 李博 郑鹏[1] WANG Hongzhan;CHEN Yanru;LI Qi;TANG Ying;LI Bo;ZHENG Peng(College of Animal Science and Technology,Northeast Agricultural University,Harbin 150030,China)

机构地区：[1]东北农业大学动物科学技术学院,哈尔滨150030

出　　处：《黑龙江动物繁殖》2024年第1期1-6,共6页Heilongjiang journal of animal reproduction

基　　金：“十四五”国家重点研发计划项目(2023YFD1300602)。

摘　　要：接受态的子宫内膜对于奶牛胚胎的成功植入至关重要。为探讨白血病抑制因子(LIF)对奶牛子宫内膜容受性的调控机制,试验将奶牛子宫内膜上皮细胞分为对照组、LIF处理组、LIF+STAT3共处理组,研究了LIF对子宫内膜容受性的作用和STAT3信号通路对奶牛子宫内膜容受性的调控。采用实时荧光定量RT-PCR和Western blot检测容受性相关基因HOXa10、VEGF和炎症相关基因TLR4、NF-κB的mRNA和蛋白表达变化。结果表明:与对照组相比,LIF处理组奶牛子宫内膜上皮细胞的容受性相关基因HOXa10、VEGF的表达量显著增加(P<0.05),炎症相关基因TLR4、NF-κB的表达量显著增加(P<0.05)。LIF+STAT3共处理组的HOXa10、VEGF的表达量与LIF处理组比较显著降低(P<0.05),与对照组无显著差异(P>0.05);TLR4、NF-κB的表达量与LIF处理组比较无显著差异(P>0.05),显著高于对照组(P<0.05)。说明LIF通过激活STAT3信号通路调控子宫内膜上皮细胞HOXa10、VEGF的表达,能够增强子宫内膜的容受性。The receptivity of the endometrium is crucial for successful embryo implantation in cows.To explore the regulatory mechanism of leukemia inhibitory factor(LIF)on the development of endometrial receptivity in cows,the bovine endometrial epithelial cells were grouped into control group,LIF group and LIF+STAT3 group to investigate the effect of LIF on endometrial receptivity and the role of the STAT3 signaling pathway in the regulation of endometrial receptivity in cows.The changes of mRNA and protein expression of receptivity-related genes HOXa10 and VEGF,and inflammation-related genes TLR4 and NF-κB were investigated with Real-time RT-PCR and Western blot.The results showed that treatment with LIF alone significantly increased the expression of receptivity-related genes HOXa10 and VEGF(P<0.05)and inflammation-related genes TLR4 and NF-κB(P<0.05)in bovine endometrial epithelial cells.The combination of LIF and STAT3 inhibitor significantly decreased the expression levels of HOXa10 and VEGF,compared to the LIF-alone treatment group(P<0.05).However,showing no significant difference compared to the control group(P>0.05).The expression levels of TLR4 and NF-κB showed no significant difference from the LIF-alone treatment group(P>0.05),however,were significantly higher than the control group(P<0.05).It could be deduced that LIF would regulate the expression of HOXa10 and VEGF in bovine endometrial epithelial cells by activating the STAT3 signaling pathway and enhance the receptivity of bovine endometrial membrane.

关 键 词：牛子宫内膜上皮细胞 容受性 胚胎附植 STAT3抑制剂 白血病抑制因子(LIF) 

分 类 号：Q344.13[生物学—遗传学]