几种抗氧化剂对马精液冷冻保存效果的影响  

Effect of adding antioxidants to the freezing medium on cryopreservation of stallion semen

作　　者：依比布拉·阿那亚提 阿依努尔·亚森[2] 罗永明[2] 团勇 玉山江[2] 齐·阿拉达尔 Yibibula Anayat;Ayinuer Yaseng;LUO Yongming;TUAN Yong;YU Shanjiang;QI Aladaer(College of Animal Science,Xinjiang Agricultural University,Urumqi 830000,China;Institute of Animal Science,Xinjiang Academy of Animal Science,Urumqi 830011,China;Institute of Yili Animal Science,Yining 835000,China)

机构地区：[1]新疆农业大学动物科学学院,乌鲁木齐830000 [2]新疆畜牧科学院畜牧研究所,乌鲁木齐830000 [3]伊犁州畜牧科学研究所,新疆伊宁835000

出　　处：《黑龙江动物繁殖》2024年第1期7-14,共8页Heilongjiang journal of animal reproduction

基　　金：马种质资源挖掘与种质创新关键技术研究项目(2023A02005-1);新疆马产业关键技术研发项目(2022A02013-1)。

摘　　要：马精液的冷冻保存效果与其他家畜相差很大,这主要是由于马精液对冷冻过程更敏感,表现出更高的氧化应激与质膜损伤率,因此,需要找出更有效的方法来提高马精液冷冻保存效果。试验选取抗氧化剂谷胱甘肽(Gsh)、牛磺酸(Tau)、维生素C、蛋氨酸(Met)和原花青素(OPC),加入精液稀释液中,用精子分析仪测定冻融后马精液的总体活力(TM)、前进式活力(PM)、快速运动精子比例(RAP)、平均路径速度(VAP)和直线性(LIN),以评估不同抗氧化剂对马精液冷冻效果的影响;进一步试验选取牛磺酸为基础抗氧化剂,组合添加其他抗氧化剂后,评测抗氧化剂组合对马精子冻融后保存效果的影响;然后,选择在精液离心前、冷冻前和冷冻解冻后添加抗氧化剂,评估不同的抗氧化剂添加程序对马精液冷冻效果的影响。结果表明:单独添加牛磺酸、维生素C、蛋氨酸、原花青素等抗氧化剂的试验组2,3,4,5马精子活力参数均有提高;不同抗氧化剂组合试验中,添加谷胱甘肽与蛋氨酸的试验组9的抗氧化效果最好,马精子活力参数指标均高于对照组,而添加牛磺酸、谷胱甘肽、蛋氨酸的试验组8的抗氧化指标多数偏低;不同时间添加抗氧化剂牛磺酸,离心前添加的试验组10的马精子活力参数最高,冷冻前添加的试验组11次之,二者均显著高于冷冻前后二次添加的试验组12(P<0.05)。说明在精液稀释液中添加牛磺酸、维生素C、蛋氨酸、原花青素等抗氧化剂是有益的,但牛磺酸与其他抗氧化剂配合没有达到很好的抗氧化效果;抗氧化剂适宜在离心前或冷冻前添加,而冷冻前后二次添加抗氧化剂可能对精子有伤害。Cryopreservation of stallion semen has not reached the level of efficiency and positive results like other domestic farm animals.This is mainly due to the greater sensitivity of stallion sperm to the freezing process,showing higher rates of oxidative stress and plasma membrane damage,which suggest a requirement for the methods of improving the efficiency of this technique in stallion.In this experiment,Glutathione(Gsh),taurine(Tau),ascorbic acid(vitamin C),methionine(Met)and proanthocyanidins(OPC)were chosen as antioxidants and added into semen dilution,respectively.The overall viability(TM),progressive viability(PM),rapid motility sperm ratio(RAP),mean path velocity(VAP)and straightness(LIN)of semen after freezing and thawing were measured by sperm analyzer to evaluate the effects of different antioxidants on the cryopreservation of stallion semen.In the second step,taurine was used as the base antioxidant and other antioxidants were added to determine the effect of the combination of antioxidants on the preservation of equine sperm after freezing and thawing.Taurine used as the base antioxidant and some of antioxidants mentioned before were added to stallion semen before centrifugation,before freezing,before freezing and after thawing,respectively,to evaluate the effect of antioxidant combination adding procedures on the preservation of equine sperm after freezing and thawing.The results showed that taurine,vitamin C,methionine,proanthocyanidins were added in the experimental group,and the motility parameters of group 2,3,4,5 equine sperm were improved.Among the different antioxidant combination tests,the antioxidant effect of glutathione and methionine was the best in test group 9,and the indexes of equine sperm motility were higher than those of control group,while the antioxidant indexes of test group 8 supplemented with taurine,glutathione and methionine were mostly low.The motility parameters of equine sperm in group 10 added before centrifugation and group 11 added before freezing were higher than those i

关 键 词：抗氧化剂 种公马 精液 冷冻保存 活性氧自由基 

分 类 号：S821.34[农业科学—畜牧学]