Targeted mutations of BnPAP2 lead to a yellow seed coat in Brassica napus L.  

作　　者：Wei Huang Ruyu Jiao Hongtao Cheng Shengli Cai Jia Liu Qiong Hu Lili Liu Bao Li Tonghua Wang Mei Li Dawei Zhang Mingli Yan 

机构地区：[1]Hunan Research Center of Heterosis Utilization in Rapeseed/Crop Research Institute,Hunan Academy of Agricultural Sciences,Changsha 410125,China [2]Hunan Key Laboratory of Economic Crops Genetic Improvement and Integrated Utilization/School of Life and Health Science,Hunan University of Science and Technology,Xiangtan 411201,China [3]Key Laboratory of Biology and Genetic Improvement of Oil Crops,Ministry of Agriculture and Rural Affairs/Oil Crops Research Institute,Chinese Academy of Agricultural Sciences,Wuhan 430062,China

出　　处：《Journal of Integrative Agriculture》2024年第2期724-730,共7页农业科学学报（英文版）

基　　金：supported by the National Natural Science Foundation of China(31971980,U19A2029);The science and technology innovation Program of Hunan Province,China(2023RC1077);the Agricultural Science and Technology Innovation Foundation of Hunan,China(2022CX55);the Scientific Research Fund of Hunan Provincial Science and Technology Department,China(2021JC0007)。

摘　　要：The yellow seed trait is preferred by breeders for its potential to improve the seed quality and commercial value of Brassica napus.In the present study,we produced yellow seed mutants using a CRISPR/Cas9 system when the two BnPAP2 homologs were knocked out.Histochemical staining of the seed coat demonstrated that proanthocyanidin accumulation was significantly reduced in the pap2 double mutants and decreased specifically in the endothelial and palisade layer cells of the seed coat.Transcriptomic and metabolite profiling analysis suggested that disruption of the BnPAP2 genes could reduce the expression of structural and regulated genes in the phenylpropanoid and flavonoid biosynthetic pathways.The broad suppression of these genes might hinder proanthocyanidin accumulation during seed development,and thereby causing the yellow seed trait in B.napus.These results indicate that BnPAP2 might play a vital role in the regulatory network controlling proanthocyanidin accumulation.

关 键 词：yellow seed BnPAP2 PROANTHOCYANIDINS CRISPR/Cas9 

分 类 号：S565.4[农业科学—作物学]