Molecular Characterization and Antibacterial Activity of a G-Type Lysozyme in Yellow Drum(Nibea albiflora)  

作　　者：XIAO Yao LI Jiacheng TIAN Qianqian WU Baolan HAN Fang 

机构地区：[1]State Key Laboratory of Mariculture Breeding,Fisheries College of Jimei University,Xiamen 361021,China

出　　处：《Journal of Ocean University of China》2024年第2期477-486,共10页中国海洋大学学报（英文版）

基　　金：supported by the National Natural Science Foundation of China(No.32072969);the National Key R&D Program of China(No.2022YFD2401002);the Natural Science Foundation of Fujian Province(No.2022 J01325);the Open Research Fund Program of Fujian Provincial Key Laboratory of Marine Fishery Resources and Eco-Environment(No.Z822280).

摘　　要：Lysozyme(EC3.2.1.17)plays an important role in the immune response;as a nonspecific immune factor,it can resist causative agents.Lysozyme can be divided into c-type and g-type in fish.In a previous study,through genome-wide association analysis,the g-type lysozyme gene,which is named NaLyg in yellow drum(Nibea albiflora),was found to be a key candidate gene for disease resistance in response to Vibrio harveyi infection.The cDNA of NaLyg was 1025 bp,including four exons and three introns,and its open reading frame(ORF)had a full-length of 582 bp,encoding 193 amino acids.NaLyg was found to be conserved during evolution through bioinformatic analyses.The NaLyg protein possessed a sugar binding domain and three catalytic sites,including Glu71,Asp84 and Asp101.Quantitative qRT-PCR results confirmed that NaLyg gene mRNA was visibly increased after V.harveyi infection.The NaLyg protein purified by prokaryotic expression killed some gram-negative bacterial pathogens by inducing cell wall destruction,including V.harveyi,Aeromonas hydrophila and Edwardsiella tarda.Moreover,the NaLyg protein killed two gram-positive bacteria,Bacillus subtilis and Staphylococcus aureus.Taken together,the experimental results suggested that the NaLyg protein of N.albiflora played an important role in fighting bacterial infections.

关 键 词：Nibea albiflora innate immunity Vibrio harveyi g-type lysozyme antibacterial activity 

分 类 号：S917.4[农业科学—水产科学]