Salinity Acclimation Induces Reduced Energy Metabolism,Osmotic Pressure Regulation and Transcriptional Reprogramming in Hypotrichida Ciliate Gastrostyla setifera  

作　　者：JI Xin BI Luping ZOU Songbao LI Wenlu JI Daode ZHANG Qianqian 

机构地区：[1]School of Ocean,Yantai University,Yantai 264005,China [2]Key Laboratory of Coastal Environmental Processes and Ecological Remediation,Yantai Institute of Coastal Zone Research,Chinese Academy of Sciences,Yantai 264003,China [3]Zhejiang Institute of Freshwater Fisheries,Huzhou 313001,China [4]Muping Coastal Environment Research Station,Yantai Institute of Coastal Zone Research,Chinese Academy of Sciences,Yantai 264117,China

出　　处：《Journal of Ocean University of China》2024年第2期539-549,共11页中国海洋大学学报（英文版）

基　　金：supported by the National Natural Science Foundation of China(Nos.32370488,42176163,31970398 and 31672251);the Youth Innovation Promotion Association of CAS(Nos.2019216 and 2022211).

摘　　要：Coastal and estuarine protists are frequently exposed to salinity undulation.While the tolerance and stress responses of microalgae to salinity have been extensively studied,there have been scarce studies on the physiological response of heterotrophic protists to salinity stressing.In this study,we investigated the physiological response of the heterotrophic ciliate Gastrostyla setifera to a salinity of 3,via a transcriptomic approach.The first transcriptome of genus Gastrostyla was obtained utilizing a group of manually isolated ciliate individuals(cells)and RNA-seq technique.The completeness of the transcriptome was verified.Differentially expressed gene(DEG)analysis was performed among the transcriptomes of G.setifera acclimated in saline water(salinity 3)and those cultured in fresh water.The results demonstrated a significant alternation in gene transcription,in which the ciliate exhibits a transcripttomic acclimation in responding salinity stressing.The up-regulated DEGs were enriched in the pathways of cytoskeleton proteins,membrane trafficking,protein kinases and protein phosphatases.These may represent enhanced functions of ion transport,stress response and cell protections.Pathways involved in energy metabolism and biosynthesis were markedly down-regulated,reflecting decreased cell activity.Particularly,we detected significantly down-regulated genes involved in several pathways of amino acid catabolism,which may lead to accumulation of amino acids in the ciliate cell.Amino acid could act as compatible solutes in the cytoplasm to maintain the osmotic balance in saline water.Overall,this work is an initial exploration to the molecular basis of the heterotrophic protist responding to salinity stressing.The result sheds light on the mechanisms of enhancement of cell protection,reduction of cell activity,and osmotic pressure regulation in ciliates acclimated to salinity.

关 键 词：salinity stress heterotrophic protist CILIATE Gastrostyla setifera transcriptome differentially expressed gene 

分 类 号：Q494[生物学—生理学]