4株生防细菌对燕麦根腐病原菌抑菌特性研究  

Inhibition characteristics of four strains of biocon⁃trol bacteria against root rot pathogens of oats

作　　者：金艳丽 姚拓[1] 兰晓君[1] 马亚春 JIN Yan-li;YAO Tuo;LAN Xiao-jun;MA Ya-chun(College of Grassland Science,Gansu Agricultural University,Key Laboratory for Grassland Ecosystem,Ministry of Education,Grassland Engineering Laboratory of Gansu Province,Sino-U.S.Centers for Grazing Land Ecosystem Sustainability,Lanzhou 730070,China)

机构地区：[1]甘肃农业大学草业学院,草业生态系统教育部重点实验室,甘肃省草业工程实验室,中-美草地畜牧业可持续发展研究中心,甘肃兰州730070

出　　处：《草原与草坪》2023年第6期9-16,共8页Grassland and Turf

基　　金：国家燕麦荞麦产业体系(CARS-08-C);现代牧草产业技术体系(CARA-35)。

摘　　要：【目的】探究3株芽孢杆菌LSH11、R1、TZF-1和1株类芽孢杆菌CCM对燕麦根腐病病原菌的抑制效果及其抑菌特性。【方法】以燕麦根腐病病原菌麦根腐平脐孺孢菌(Bipolaris sorokiniana)和串珠赤霉菌(Gibberella moniliformis)为靶标菌,利用十字交叉法测定4株生防细菌的抑菌能力;采用透明圈法测定菌株产蛋白酶、纤维素酶及其淀粉酶的能力;采用结晶紫染色检测菌株形成生物膜的能力,利用分子生物学方法检测菌株是否具有合成相关脂肽类物质的基因。【结果】4株生防细菌对两种病原菌均具有较好的抑制效果,抑制率达53%以上,并导致真菌菌丝出现异常断裂、分枝、畸形、内含物质外泄的现象。4株菌均具备产蛋白酶、淀粉酶和纤维素酶的能力,并且均具有形成生物膜的能力。扩增脂肽物质相关基因(ituA、ituB、ituC、ituD、fenA、fenD、srfAA)发现3株芽孢杆菌均具有脂肽类物质合成的相关基因。【结论】4株生防细菌可分泌水解酶等抑菌类物质,具有良好的应用潜力,其中菌株R1和TZF-1综合生防特性较好,可用于后期生防菌肥的制作和燕麦根腐病的生物防治。【Objective】This research aimed to investigate the inhibitory effect and characteristics of three strains of Bacillus LSH11,R1,TZF⁃1 and one strain of Bacillus cereus CCM on the root rot pathogen of oats.【Method】The oat root rot pathogens(Bipolaris sorokiniana and Gibberella moniliformis)were selected as target bacteria to determine the bacterial inhibitory capacity of four strains of biocontrol bacteria using the crossover method.The abil⁃ity of the strains to produce protease,cellulase and its amylase was assessed using the clear circle method.The ability of the strains to form biofilm was detected using crystalline violet staining,and molecular biological methods were em⁃ployed to ascertain whether the strains possessed genes for the synthesis of related lipopeptide substances.【Result】The results showed that allfour strains of biocontrol bacteria exhibited a significant inhibitory effect on both patho⁃gens,with an inhibition rate of 53%.This inhibition led to abnormal fracture of fungal mycelium,manifested as branching,deformation,and leakage of inner material.All four strains demonstrated the ability to produce protease,amylase and cellulase,and they all exhibited biofilm⁃forming capabilities.Amplification of lipopeptide⁃related genes(ituA,ituB,ituC,ituD,fenA,fenD,srfAA)revealed that all three Bacillus strains possessed genes related to the syn⁃thesis of lipopeptides.【Conclusion】The four strains of antibacterial bacteria demonstrated the secretion of antibacte⁃rial substances such as hydrolytic enzymes,which have great potential for application.Notably,strains R1 and TZF⁃1 exhibited superior integrated biocontrol characteristics,making them suitable for the production of late stage biocon⁃trol fertilizer and biological control of oat root rot.

关 键 词：生防细菌 燕麦根腐病 抑真菌特性 

分 类 号：S435.126[农业科学—农业昆虫与害虫防治]