鸡蛋过敏患儿蛋清4种组分sIgE抗体分布特征  

Distribution characteristics of sIgE antibodies against four components of egg white in children with egg allergy

作　　者：孙瑗敏 谭欣 郑力胜 张蓓 杨小慧 于洋[1] 李会强[1] SUN Yuanmin;TAN Xin;ZHENG Lisheng;ZHANG Bei;YANG Xiaohui;YU Yang;LI Huiqiang(College of Medical Laboratory,Tianjin Medical University,Tianjin 300203;Department of Medical Laboratory,Tianjin Children′s Hospital,Tianjin 300074,China)

机构地区：[1]天津医科大学医学检验学院,天津300203 [2]天津市儿童医院检验科,天津300074

出　　处：《临床检验杂志》2024年第1期1-5,共5页Chinese Journal of Clinical Laboratory Science

基　　金：国家自然科学基金面上项目(81772259)。

摘　　要：目的分析鸡蛋过敏患儿血清中蛋清4种过敏原蛋白组分的sIgE抗体分布特征,明确鸡蛋过敏单组分解析诊断的临床应用价值。方法收集197例鸡蛋过敏儿童血清样本,采用光激化学发光法检测蛋清中4种主要过敏原组分蛋白(卵类黏蛋白、卵白蛋白、卵转铁蛋白、溶菌酶)sIgE抗体的水平,并分析不同组分sIgE抗体的分布特征。结果152例(77.16%)患儿血清中含有卵白蛋白sIgE抗体,139例(70.56%)含有卵类黏蛋白sIgE抗体,69例(35.02%)含有卵转铁蛋白sIgE抗体,但仅有36例(18.27%)患儿血清中含有溶菌酶sIgE抗体。30.45%的患儿血清中同时含有针对卵类黏蛋白和卵白蛋白的sIgE抗体;鸡蛋sIgE抗体含量与蛋清4种组分sIgE抗体累计含量的相关性不显著(r=0.2668,P<0.05),且不同患儿血清中蛋清4种组分sIgE抗体的含量存在明显的个体差异。结论患儿血清中蛋清4种组分sIgE抗体含量存在个体间异质性,蛋清组分sIgE抗体检测可以区分不同形式的鸡蛋过敏,对于儿童鸡蛋过敏的精准诊断与精准脱敏治疗具有重要的价值。Objective To investigate the distribution characteristics of serum sIgE antibodies against four allergenic protein components of egg white in children with egg allergy,and then clarify the clinical application value of single component-resolved diagnostics of egg allergy.Methods Serum samples from 197 children with egg allergy were collected.The levels of serum sIgE antibodies against four major allergenic protein components of egg white,including ovomucin,ovalbumin,ovotransferrin,and lysozyme,were detected by the light-excited chemiluminescence assay(LiCA),and the distribution characteristics of sIgE antibodies were analyzed.Results The positive rates of serum sIgE antibodies against ovalbumin,ovomucin,ovotransferrin,and lysozyme in 197 chlidren with egg allergy were 77.16%(152/197),70.56%(139/197),35.02%(69/197),and 18.27%(36/197),respectively.The positive rate of serum sIgE antibody against both ovomucin and ovalbumin was 30.45%.There was a weak correlation between the levels of sIgE antibodies against egg and the cumulative levels of sIgE antibodies against four allergenic protein components(r=0.2668,P<0.05).There were significant individual differences in the levels of serum sIgE antibodies against four allergenic protein components of egg white in the children with egg allergy.Conclusion There is individual heterogeneity in the levels of serum sIgE antibodies against four components of egg white in the children with egg allergy.The detection of sIgE antibodies against egg white components can distinguish different forms of egg allergies,which is of great value for the accurate diagnosis and precise desensitization of children′s egg allergy.

关 键 词：鸡蛋过敏 组分诊断 蛋清过敏原组分 特异性IGE抗体 分布特征 

分 类 号：R446.6[医药卫生—诊断学]