普通小麦Arableu#1白粉病成株抗性遗传解析  

Genetic Analysis of Adult Plant Resistance to Powdery Mildew in Common Wheat Arableu#1

作　　者：李子萌 袁婵 张宇庆[2] 任妍[3] 刘鹏鹏[4] 严珊珊 袭梦涵 穆培源[4] 兰彩霞[1] LI ZiMeng;YUAN Chan;ZHANG YuQing;REN Yan;LIU PengPeng;YAN ShanShan;XI MengHan;MU PeiYuan;LAN CaiXia(College of Plant Science and Technology,Huazhong Agricultural University/Hubei Hongshan Laboratory,Wuhan 430070;Institute of Food Crops,Hubei Academy of Agricultural Sciences,Wuhan 430072;College of Agronomy,Henan Agricultural University,Zhengzhou 450002;Institute of Crop Research,Xinjiang Academy of Agricultural and Reclamation Science,Shihezi 832000,Xinjiang)

机构地区：[1]华中农业大学植物科学技术学院/湖北洪山实验室,武汉430070 [2]湖北省农业科学院粮食作物研究所,武汉430072 [3]河南农业大学农学院,郑州450002 [4]新疆农垦科学院作物研究所,新疆石河子832000

出　　处：《中国农业科学》2024年第1期52-64,共13页Scientia Agricultura Sinica

基　　金：湖北洪山实验培育项目(2022hspy001);国家自然科学基金国际合作项目(31861143010)。

摘　　要：【目的】中国是世界上最重要的小麦生产国和消费国之一,确保小麦产量稳定对我国粮食安全具有重要意义。小麦白粉病是限制小麦产量的重要因素之一。选育抗病品种是控制该病害最经济、有效环保的途径,挖掘新成株抗性基因/位点为选育持久抗病小麦品种提供依据。【方法】由国际玉米小麦改良中心(CIMMYT)培育的普通小麦品系Arableu#1在苗期对白粉病表现为感病,而成株期呈现高水平抗性。为深入解析其抗病遗传机制,利用Arableu#1与高感品系Apav#1杂交,构建一个含有142个家系的F5重组自交系群体,采用基因分型测序(genotyping-by-sequencing,GBS)平台测序,获得4298个在亲本间具有多态性的分子标记,并进行群体检测,所获基因型用于构建整个群体的遗传连锁图。【结果】结合白粉病连续2年田间表型和基因型数据,使用IciMapping4.1对田间病害严重度、病程曲线下面积(AUDPC)和病害多年平均严重度(MFDS)等性状进行QTL定位分析,在该群体中定位了4个来自Arableu#1的白粉病成株抗性位点,分别位于染色体1BL、5BL、6BS和7BL上,以及2个来自Apav#1的抗性位点,分别位于染色体2DS和4BL上,可解释白粉病表型变异介于3.1%—28.5%。6个位点聚合时的群体平均病害严重度达到各位点组合中的最低水平,QPm.hzau-1BL、QPm.hzau-4BL、QPm.hzau-5BL和QPm.hzau-7BL4个位点聚合后的效应仅次于6个位点同时存在。【结论】将2个新位点QPm.hzau-5BL和QPm.hzau-7BL的两翼分子标记开发成KASP标记,经对570份世界各地育成品种或地方品种检测,2个位点单独存在或聚合在一起时均表现出不同程度的白粉病抗性,且这两个位点在我国小麦材料中的分布频率较CIMMYT的小麦种质中低。获得1个可能同时聚合6个白粉病抗性位点家系,其田间白粉病抗性可达近免疫水平。【Objective】China is a significant wheat producer and consumer in the world.It is very important for our food security to making sure the stable wheat production.Wheat powdery mildew is an important leaf disease to affect the global wheat yield.Breeding resistant wheat varieties is one of the most economically and environmentally effective way to manage this disease.Identification of new adult plant resistant loci is very important for breeders to develop durable powdery mildew-resistant wheat varieties.【Method】The CIMMYT-derived common wheat(Triticum aestivum L.)Arableu#1,showed susceptibility to powdery mildew at the seedling stage,but exhibited a high level of resistance to this disease at the adult plant stage in the field.In order to understand the genetic basis of powdery mildew resistance in this line,we made an F5 recombinant inbred line(RIL)population between Arableu#1 and the susceptible line Apav#1 and genotyped by genotyping-by-sequencing(GBS)platform.We obtained 4298 polymorphic molecular markers between the parents and analyze the 142 RILs.Joinmap 4.1 and IciMapping 4.1 were respectively used to construct the linage maps and identify the powdery mildew resistance QTL in this study.【Result】Four adult plant resistance loci from Arableu#1 were identified on wheat chromosome arms 1BL,5BL,6BS and 7BL,while two loci from Apav#1 on chromosomes 2DS and 4BL,which can explain the powdery mildew disease severity variation from 3.1%to 28.5%.The MFDS of RIL reached the lowest level when it carried all six detected resistance loci,while RILs combing QPm.hzau-1BL,QPm.hzau-4BL,QPm.hzau-5BL,and QPm.hzau-7BL also showed lower disease severity over multiple environments.【Conclusion】KASP markers KASP-5B-1 and KASP-7B-2 for QPm.hzau-5BL and QPm.hzau-7BL,respectively,were developed in the present study.The two loci showed varying degrees of resistance to powdery mildew when present individually or in combination based on the genotype and phenotype of 570 wheat germplasm collections.Additionally,the frequen

关 键 词：小麦 白粉病 成株抗性 数量性状 基因定位 

分 类 号：S435.12[农业科学—农业昆虫与害虫防治]