北京月季叶际表生真菌多样性及群落结构  

Diversity and community structure of epiphytic fungi in the phyllosphere of Rosa chinensis in Beijing,northern China

作　　者：崔玉进 吴芳[1] CUI Yujin;WU Fang(Institute of Microbiology,School of Ecology and Nature Conservation,Beijing Forestry University,Beijing 100083,China)

机构地区：[1]北京林业大学生态与自然保护学院微生物研究所,北京100083

出　　处：《菌物学报》2024年第1期1-18,共18页Mycosystema

摘　　要：叶际微生物在维持植物健康,促进植物的生长和生物地球化学循环等生态功能中发挥着重要作用,近年来叶际微生物的作用以及多样性变化受到广泛关注,其重要性日益突出。本研究以北京常见的绿化植物月季为研究对象,对市中心向北、由近到远的4个采样点,安定门地区(ADM)、和平街地区(HPJ)、亚运村地区(YYC)和北七家地区(BQJ);3个月份(四月、七月和十一月)和道路2个不同位置(道路中央和两侧)的月季叶际表生真菌多样性及群落结构进行了研究。结果表明:在月季叶际表生真菌系统中,共获得3425条扩增子系列变异特征序列(ASVs),其中座囊菌纲Dothideomycetes是优势类群,不同采样点间的月季叶际表生真菌Shannon指数由高到低依次为ADM>BQJ>HEJ>YYC,其中ADM显著高于YYC,多样性由市中心向北、由近到远呈现先降低后升高的变化趋势;月份间的Shannon指数由高到低依次为四月份>七月份>十一月份,其中四月份显著高于七月份和十一月份;道路中央样品与道路两侧样品无显著性差异。LEfSe分析判定了不同地区和季节的差异指示菌属,其中ADM的差异指示属为新刺毛茎点霉属Neosetophoma、新齿裂菌属Neophaeococcomyces及假赤壳属Pseudocosmospora,HPJ的差异指示属为光黑壳属Preussia,YYC的差异指示属为圆盘菌属Orbilia,BQJ的差异指示属为假小垫革菌属Pseudotomentella。通过网络分析发现,空泡茎点霉属Vacuiphoma、担孢酵母属Erythrobasidium、枝孢属Cladosporium、木拉克属Mrakia和亚球壳属Sphaerulina平均度最大,确定为核心节点菌属。真菌群落功能预测得出腐生功能类群和病理功能类群是月季叶际表生真菌的主要类群,且腐生功能类群在不同季节条件下和不同样点间丰度均存在显著变化。研究结果为城市叶际微生物群落结构变化和城市绿化科学管理提供了参考。Phyllosphere microorganisms play important roles in ecological functions,such as maintaining plant health,promoting plant growth and biogeochemical cycling.In recent years,the role and importance of phyllosphere microbes have received increasing attention.The diversity and community structure of epiphytic fungi in the phyllosphere of Rosa chinensis,a common greening plant in Beijing,were investigated.Four sites from the center to north of the city and near to far distance,Andingmen area(ADM),Hepingjie area(HPJ),Yayuncun area(YYC),and Beiqijia area(BQJ),were selected.The samples were taken in three seasons(April,July,and November)at different locations(the middle and sides of the road of the selected areas).A total of 3425 amplicon sequence variants(ASVs)was obtained,and they were dominated by Dothideomycetes.The variance of Shannon index between sampling sites were ADM>BQJ>HPJ>YYC,indicating ADM is significantly higher than YYC.The diversity shows a decreasing and then increasing trend from the center to north of the city.The variance of Shannon index between seasons showed April>July>November,and April was significantly higher than July and November.There is no significant difference between the samples from the center and the sides of road.LEfSe analysis suggested the significant biomarkers of different regions and seasons.Significant biomarkers of Andingmen area were Neosetophoma,Neophaeococcomyces and Pseudocosmopora,while the significant biomarkers of Hepingjie area,Yayuncun area and Beiqijia area were Preussia,Orbilia and Pseudotomentella,respectively.Network analysis showed the dominant genera were Vacuiphoma,Erythrobasidium,Cladosporium,Mrakia,and Sphaerulina.The main functional groups of epiphytic fungi in the phyllosphere of Rosa chinensis were pathotroph and saprotroph.The prediction of fungal community function demonstrates that saprophytic functional groups were different in different seasons and sampling sites.The study revealed structural changes of phyllosphere fungi in urban plants and might be v

关 键 词：叶际真菌 月季 Illumina MiSeq测序 真菌多样性 群落组成 指示物种 

分 类 号：S685.12[农业科学—观赏园艺]