堀刺盘孢效应蛋白Ch4839的功能初探及其互作蛋白筛选  

Function and interacting proteins of effector Ch4839 in Colletotrichum horii

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作  者:丁瑜 李家艳 范芝蕊 吕中一 杨勇[1] 关长飞 DING Yu;LI Jiayan;FAN Zhirui;LÜZhongyi;YANG Yong;GUAN Changfei(College of horticulture,Northwest A&F University,Yangling 712100,Shaanxi,China)

机构地区:[1]西北农林科技大学园艺学院,陕西杨凌712100

出  处:《菌物学报》2024年第1期79-89,共11页Mycosystema

基  金:国家自然科学基金面上项目(32272672)。

摘  要:柿炭疽病是影响柿树栽培及产业发展的主要病害之一,我国柿炭疽病的主要真菌病原菌为堀刺盘孢Colletotrichumhorii。效应蛋白是一类由病原菌分泌用于诱导宿主防御反应或抑制宿主免疫反应的蛋白质,然而柿堀刺盘孢效应蛋白的相关报道较少。本研究基于课题组报道的堀刺盘孢基因组信息,获得了效应蛋白Ch4839并对其进行初步功能分析。结果表明:与野生型堀刺盘孢相比,Ch4839的敲除突变体ΔCh4839-4-1并不影响堀刺盘孢的菌落生长,但是产孢率与野生型相比有所减少;且Ch4839敲除突变体的致病力明显下降;亚细胞定位显示该效应蛋白可以定位于烟草的细胞核、细胞膜以及部分细胞质上;此外,Ch4839可以抑制由INF1引发的烟草细胞程序性凋亡,表明该效应蛋白在抑制宿主防卫作用中发挥作用;进一步通过酵母文库筛选获得了5个候选靶蛋白。试验结果初步揭示效应蛋白Ch4839的功能,为探究柿炭疽病效应蛋白与宿主靶蛋白之间的互作机理提供理论依据。Persimmon anthracnose is one of the main diseases affecting cultivation and industrial development of persimmon.The main fungal pathogen of persimmon anthracnose in China is Colletotrichum horii.The effector is a type of secreted protein by pathogenic microbes to induce or inhibit host defense response.At present,there are few studies on the effector of C.horii.In this study,the effector Ch4839 was screened from the genome of C.horii,and its function and target protein(s)screened were analyzed.The results showed thatΔCh4839-4-1,the knockout mutant of Ch4839,did not affect the colony growth of C.horii but the sporulation rate was reduced.The pathogenicity of the knockout mutant decreased significantly.Subcellular localization showed that the effector was localized in the nucleus,cell membrane and some cytoplasm of tobacco.In addition,Ch4839 exhibited the inhibition of the programmed cell death of tobacco-induced by INF1,indicating that the effector possessed a role in inhibiting host defense.There were 5 candidate target proteins obtained by yeast library screening.The results preliminarily reveal the function of the effector Ch4839,and further exploration of the interaction mechanism between the effector and the host target protein is needful.

关 键 词:Colletotrichum horii 柿炭疽病 效应蛋白敲除 酵母双杂交 

分 类 号:S436.65[农业科学—农业昆虫与害虫防治]

 

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