HIV-1感染患者外周血单核细胞miR-20a、miR-122表达与HIV-1 DNA、HIV-1 RNA及CD4+T淋巴细胞计数的关系  

The relationship between the expression of miR-20a and miR-122 and the count of HIV-1 DNA,HIV-1 RNA and CD4+T lymphocytes in peripheral blood mononuclear cells of patients infected with HIV-1

作　　者：许翼 刘超[2] 蒋华春 计然然 匡宁 Xu Yi;Liu Chao;Jiang Huachun(Department of Clinical Laboratory,Yixing Traditional Chinese Medicine Hospital,Wuxi 214200,China)

机构地区：[1]宜兴市中医医院检验科,无锡214200 [2]南通市第三人民医院检验科

出　　处：《中华保健医学杂志》2023年第6期702-706,共5页Chinese Journal of Health Care and Medicine

基　　金：宜兴市科技发展项目(SKJYD2021011)。

摘　　要：目的检测人类免疫缺陷病毒-1(HIV-1)感染者外周血单个核细胞(PBMCs)微小RNA(miR)-20a、miR-122表达水平,探讨其与HIV-1 DNA、HIV-1 RNA及CD4+T淋巴细胞计数关系。方法选取2021年5月~2023年1月南通市第三人民医院收治的HIV-1感染患者110例作为HIV-1组,依据HIV-1 DNA分为HIV-1 DNA高表达组(DHG组,n=65例)、HIV-1 DNA低表达组(DLG组,n=45例);依据HIV-1 RNA分为HIV-1 RNA高表达组(RHG组,n=49例)、HIV-1 RNA低表达组(RLG组,n=61例);根据CD4+T淋巴细胞计数分为<200 cellsμl组(n=36例)、200~350 cellsμl组(n=48例)、>350 cellsμl组(n=26例)。另选取同时间段健康体检者110例作为健康对照组。荧光定量PCR法检测PBMCs miR-20a、miR-122、HIV-1 DNA以及血浆HIV-1 RNA,流式细胞仪获取CD4+T淋巴细胞计数。比较各组患者PBMCs中miR-20a、miR-122水平差异。Pearson法分析两指标间相关性。结果HIV-1组PBMCs中miR-20a、miR-122水平高于健康对照组[(1.72±0.45)vs.(1.09±0.12)、(1.51±0.38)vs.(1.04±0.07)],差异有统计学意义(t=14.188、12.757,P<0.05)。与DLG组相比,DHG组患者PBMCs中miR-20a、miR-122水平均明显升高[(1.91±0.48)vs.(1.45±0.41)、(1.64±0.39)vs.(1.32±0.37)],差异有统计学意义(t=5.239、4.320,P<0.05)。RHG组PBMCs中miR-20a、miR-122水平高于RLG组[(1.85±0.46)vs.(1.62±0.44)、(1.70±0.40)vs.(1.36±0.36)],差异有统计学意义(t=2.670、4.685,P<0.05)。<200 cellsμl组、200~350 cellsμl组、>350 cellsμl组miR-20a、miR-122水平逐次降低[(2.04±0.55)、(1.67±0.46)、(1.37±0.29),(1.73±0.40)、(1.51±0.39)、(1.21±0.33)],差异有统计学意义(F=16.525,14.117,P<0.05)。相关性结果显示,HIV-1组PBMCs中miR-20a与miR-122呈正相关(r=0.651,P<0.05),miR-20a、miR-122均与HIV-1 DNA(log 2 DNA)有明显正相关(r=0.569、0.471,P<0.05),与HIV-1 RNA(log 2 RNA)有明显正相关(r=0.491、0.444,P<0.05),但与CD4+T淋巴细胞计数呈负相关性(r=-0.555、-0.536,P<0.05)。结论HIV-1感染者PBMCs中miR-20a、miR-122表达水平升高,与CDObjective The expression levels of micrornas(miR-20a)and miR-122 in peripheral blood mononuclear cells(PBMCs)infected with human immunodeficiency virus 1(HIV-1)were detected to investigate their relationship with HIV-1 DNA,HIV-1 RNA and CD4+T lymphocyte count.Methods A total of 110 HIV-1 infected patients admitted to Nantong third people′s hospital from May 2021 to January 2023 were selected as the HIV-1 group.According to HIV-1 DNA,they were divided into HIV-1 DNA high expression group(DHG group,65 cases)and HIV-1 DNA low expression group(DLG group,45 cases).According to HIV-1 RNA,they were divided into HIV-1 RNA high expression group(RHG group,49 cases)and HIV-1 RNA low expression group(RLG group,61 cases).According to the CD4+T lymphocyte count,they were grouped into<200 cellsμl group(36 cases),200~350 cellsμl group(48 cases),and>350 cellsμl group(26 cases).Another 110 healthy subjects in the same period were selected as the control group.Fluorescence quantitative PCR was applied to detect PBMCs miR-20a,miR-122,HIV-1 DNA,and plasma HIV-1 RNA,the CD4+T lymphocyte count was obtained by flow cytometry.The differences in the levels of miR-20a and miR-122 in PBMCs of patients in each group were compared.Pearson method was applied to analyze the correlation between the two indicators.Results The levels of miR-20a and miR-122 in PBMCs of HIV-1 group were higher than those of control group[(1.72±0.45)vs.(1.09±0.12),(1.51±0.38)vs.(1.04±0.07)](t=14.188,12.757,P<0.05).Compared with DLG group,the levels of miR-20a and miR-122 in PBMCs of HIV-1 group in DHG group were obviously higher[(1.91±0.48)vs.(1.45±0.41),(1.64±0.39)vs.(1.32±0.37)](t=5.239,4.320,P<0.05).The levels of miR-20a and miR-122 in PBMCs of HIV-1 group in RHG group were higher than those in RLG group[(1.85±0.46)vs.(1.62±0.44),(1.70±0.40)vs.(1.36±0.36)](t=2.670,4.685,P<0.05).The levels of miR-20a and miR-122 in the groups of<200 cellsμl,200~350 cellsμl and>350 cellsμl decreased gradually[(2.04±0.55),(1.67±0.46),(1.37±0.29),(1.73±0.40),(1

关 键 词：人类免疫缺陷病毒-1 单个核细胞 微小RNA-20a 微小RNA-122 

分 类 号：R593.3[医药卫生—内科学]