4株虫生真菌对亚洲玉米螟致病力评价及其与松毛虫赤眼蜂的相容性  

Pathogenicity Evaluation of Four Entomopathogenic Fungi against Ostrinia furnacalis and Their Compatibility with Trichogramma dendrolimi

作　　者：赵宇[1] 姜媛媛[1] 田艺帆 王佳江[1] 隋丽[1] 张云月[1] 李启云[1,2,3] 路杨 张正坤[1] ZHAO Yu;JIANG Yuan-yuan;TIAN Yi-fan;WANG Jia-jiang;SUI Li;ZHANG Yun-yue;LI Qi-yun;LU Yang;ZHANG Zheng-kun(Jilin Academy of Agricultural Sciences,Changchun 130033;College of Plant Protection,China Agricultural University,Beijing 100193;Jilin Agriculture Science and Technology University,Jilin 132101,China)

机构地区：[1]吉林省农业科学院,长春130033 [2]中国农业大学植物保护学院,北京100193 [3]吉林农业科技学院,吉林132101

出　　处：《玉米科学》2023年第6期135-142,共8页Journal of Maize Sciences

基　　金：吉林省农业科技创新工程重大项目(CXGC2021ZD008);吉林省科技发展计划项目(20220202021NC、20230302001NC、20200702037NC)。

摘　　要：利用球孢白僵菌与赤眼蜂联合控制亚洲玉米螟已被证明具有较好的协同增效防治效果,但未见其他昆虫病原真菌与赤眼蜂共同施用下的相容性研究报道。本研究从田间采集的斜纹夜蛾幼虫僵虫中分离并鉴定出4株对亚洲玉米螟具有较高毒力的昆虫病原真菌菌株,分别为诺米曲霉(Aspergillus nomiae)、木贼镰孢(Fusarium equiseti)、莱氏绿僵菌(Metarhizium rileyi)和糙孢蓝状菌(Talaromyces trachyspermus),对亚洲玉米螟2龄幼虫半致死时间(LT50)分别为4.37、4.11、3.69和5.19 d。采用0.1%(w/v)的淀粉溶液作为吸附助剂可实现赤眼蜂单头蜂载菌量超过3.29万个孢子。进一步研究发现,这4种昆虫病原真菌对松毛虫赤眼蜂羽化及其对亚洲玉米螟虫卵寄生性无明显影响。Combined application of B.bassiana with Trichogramma was used to control the Asian corn borer(ACB,Ostrinia furnacalis) has been proved to be systemic.However,there are few reports on compatibility of the other Entomopathogenic fungus(EPF) with T.dendrolimi,which was important for systemic control of ACB.In this study,four EPF strains were isolated and identified from naturally infected cadavers of a larvae of Spodoptera litura in soybean field,which were Aspergillus nomiae,Fusarium equiseti,Metarhizium rileyi and Talaromyces trachyspermus,respectively.The pathogenicity against ACB larvae was evaluated,with the median lethal time(LT50) values of4.37 d,4.11 d,3.69 d,and 5.19 d,respectively.Using 0.1%(w/v) starch solution as an adsorbent,the conidia of four fungal species load on T.dendrolimi were all more than 32 900 spores per wasp.Further study showed that the four EPF strains showed no significant effect on T.dendrolimi emergence and egg parasitism of ACB.

关 键 词：玉米 亚洲玉米螟 昆虫病原真菌 赤眼蜂 生物防治 

分 类 号：S435.131[农业科学—农业昆虫与害虫防治]