当归痛风方对小鼠高尿酸血症肾病的FTIR研究  

FTIR Study on the Effect of DangGuiTongFengFang on Hyperuricemia Nephropathy in Mice

作　　者：刘必旺 路荣荣 曹越 王秀文 赵换 郝渺 马晓霞 马艳苗 王永辉 LIU Bi-wang;LU Rong-rong;CAO Yue;WANG Xiu-wen;ZHAO Huan;HAO Miao;MA Xiao-xia;MA Yan-miao;WANG Yong-hui(FUSHAN College,Shanxi University of Chinese Medicine,Yuci 030619,China;Basic Medicine College,Shanxi University of Chinese Medicine,Yuci 030619,China;College of TCM and Food Engineering,Shanxi University of Chinese Medicine,Yuci 030619,China;Pharmacy Department,Affiliated Hospital of Shanxi University of Chinese Medicine,Taiyuan 030024,China)

机构地区：[1]山西中医药大学傅山学院,山西榆次030619 [2]山西中医药大学基础医学院,山西榆次030619 [3]山西中医药大学中药与食品工程学院,山西榆次030619 [4]山西中医药大学附属医院药剂科,山西太原030024

出　　处：《光谱学与光谱分析》2024年第3期800-806,共7页Spectroscopy and Spectral Analysis

基　　金：国家自然科学基金重点支持项目(U21A20410);山西中医药大学科技创新团队(2022TD2005);山西省高等学校大学生创新创业训练计划项目(20220718);山西省自然科学研究面上项目(202203021211217)资助。

摘　　要：傅里叶变换红外光谱(FTIR)是鉴别物质和分析物质结构的有效手段,可对样品进行定性和定量分析,广泛应用于医药化工等众多领域。FTIR作为一种操作简便、实用、准确率高和费用低的鉴别方法,如今已经普遍应用于各种药品检验中,而有关其应用于药理研究中还非常少,目前仅有采用红外光谱分析法对各种肿瘤组织进行研究的报道。高尿酸血症肾病是人体血尿酸升高后,过高的尿酸负荷超过肾脏的清除能力,尿酸结晶沉积于集合管、肾盂和尿道等。采用FTIR技术评价高尿酸血症肾病模型,并评价药物的药效,具有一定的可行性。该实验为探讨FTIR评价高尿酸血症肾病模型和当归痛风方疗效的科学性,将60只KM小鼠随机分为6组,正常对照组、模型组、阳性对照组和当归痛风方低、中、高组,采用氧嗪酸钾复制高尿酸血症肾病模型,灌胃当归痛风方,检测血清UA、尿液UA、血清CRE、尿液CRE、血清IL-1β和肾脏IL-1β、肾脏FTIR谱图等,并观察肾脏的病理变化。结果表明模型组各项指标与正常组比较有统计学意义(p<0.05),当归痛风方高剂量组的血清UA、血清CRE、血清IL-1β和肾脏IL-1β等与模型组比较且有统计学意义(p<0.05)。当归痛风方高剂量组肾脏的病理变化有所改善,无炎性细胞浸润,管腔内无管型,说明对肾损伤具有保护作用。各组FTIR谱有明显的差异,模型组的强度均低于正常组,阳性对照组和当归痛风方高剂量组谱接近正常组,进一步说明当归痛风方对肾损伤具有保护作用。研究表明FTIR可以作为一种简便快捷灵活经济的评价动物模型及药物疗效的方法之一,也为高尿酸血症肾病模型及有效药物的筛选提供了一个有意义的技术手段。该实验拓展了FTIR检测方法的使用范围。The Fourier transform infrared spectrometer(FTIR)is an effective method to identify and analyze the structure of substances.It can be used to characterize Quantitative analysis samples and is widely used in many fields,such as medicine and the chemical industry.FTIR,as a simple,practical,accurate and low-cost identification method,has been widely used in drug testing,but its application in pharmacological research is very limited.There are only reports on the study of various kinds of tumor tissues by infrared spectrum analysis.Hyperuricemia nephropathy is a condition in which a high uric acid load exceeds the ability of the kidneys to clear the elevated level of serum uric acid.The uric acid crystals are deposited in collecting ducts,the renal pelvis and the urethra.Therefore,it is feasible to use FTIR to evaluate the Hyperuricemia nephropathy model and evaluate the efficacy of drugs.To explore the feasibility of FTIR in evaluating the Hyperuricemia nephropathy model and the therapeutic effect of DangGuiTongFengFang,60 km mice were randomly divided into 6 groups,the Rats in the normal control group,model group,positive control group and the low,middle and high groups of the Chinese angelica gouty prescription were treated with potassium oxonateto make the model of Hyperuricemia nephropathy,serum UA,urine UA,serum CRE,urine CRE,serum IL-1β,renal IL-1βand FTIR spectra were detected.The results showed that the indexes of the model group were significantly different from those of the normal group(p<0.05),and the serum levels of UA,CRE,IL-1βand renal IL-1βin the high-dose group were also significantly higher than those in the model group(p<0.05).The pathological changes of the kidney in the high-dose group were improved,with no inflammatory cell infiltration and no tubular type in the lumen,indicating that the high-dose group has a protective effect on the kidney injury.The FTIR spectra of the model group were lower than that of the normal group.The FTIR spectra of the positive control group and the high-dose gro

关 键 词：高尿酸血症肾病 当归痛风方 尿酸 肌酐 傅里叶变换红外光谱仪 

分 类 号：R285.5[医药卫生—中药学]