云杉花墨天牛转录组分析及其生物防治相关基因筛选  

Transcriptome study and screening of biocontrol related genes in Sakhalin pine sawyer Monochamus saltuarius

作　　者：方思茗 徐周策 吴宗仁 石娟 Fang Siming;Xu Zhouce;Wu Zongren;Shi Juan(Sino-France Joint Laboratory for Invasive Forest Pests in Eurasia,Beijing Key Laboratory for Forest Pest Control,College of Forestry,Beijing Forestry University,Beijing 100083,China;Jiangxi Forest Pests Control and Quarantine Center,Nanchang 330006,Jiangxi Province,China)

机构地区：[1]北京林业大学林学院,林木有害生物防治北京市重点实验室,中法欧亚森林入侵生物联合实验室,北京100083 [2]江西省林业有害生物防治检疫中心,南昌330006

出　　处：《植物保护学报》2023年第6期1610-1616,共7页Journal of Plant Protection

基　　金：江西省林业厅林业科技创新专项(201912);中央高校基本科研业务费专项资金(QNTD202304)。

摘　　要：为明确云杉花墨天牛Monochamus saltuarius的基因组功能信息,并筛选其生物防治相关基因,利用Illumina Novaseq 6000平台对云杉花墨天牛成虫进行转录组测序和生物信息学分析,并在NR、GO和KEGG数据库进行基因功能注释。结果显示,共获得190.06 Gb的云杉花墨天牛有效转录组数据,得到35728个unigene,平均长度为1270.83 bp,组装得到58142个转录本,N50长度为2135 bp。所得unigene在NR数据库中比对注释到相似基因数量占比最高的物种为光肩星天牛Anoplophora glabripennis,达到63.83%,在GO数据库中按功能注释分为生物学进程、细胞组分和分子功能3大类60个亚类,在KEGG数据库注释到44条代谢通路中。根据基因注释信息进一步挖掘出具有生物防治潜力的嗅觉相关基因43个、蜕皮相关基因149个、几丁质代谢相关基因35个、飞行相关基因6个和木质纤维素相关基因39个,可作为后期开发新的分子工具和云杉花墨天牛生物防治技术的备选靶标基因。To clarify the functional information of the Sakhalin pine sawyer Monochamus saltuarius genome and identify the genes related to its biocontrol,transcriptome sequencing and bioinformatics analysis were performed on adult M.saltuarius by using the IIumina Novaseq 6000 platform.Functional annotation of the genes was carried out in the NR,GO and KEGG databases.The results revealed a total of 190.06 Gb of validated transcriptome data,yielding 35728 unigenes for M.saltuarius.The average unigene length was 1270.83 bp,with 58142 transcripts and an N50 length of 2135 bp.Comparison and annotation of the unigenes in the NR database showed the highest percentage of similar genes in Anoplophora glabripennis(63.83%).Functional annotation in the GO database classified unigenes into three major categories and 60 subcategories:biological processes,cellular components,and molecular functions.In the KEGG database,44 KEGG metabolic pathways were annotated.Based on the gene annotation information,43 olfaction-related genes,149 molt-related genes,35 genes related to chitin metabolism,six flight-related genes,and 39 genes related to lignocellulose were identified.These genes could serve as potential target genes for the development of new molecular tools and biocontrol technologies for M.saltuarius.

关 键 词：云杉花墨天牛 转录组 靶标基因 生物信息学分析 生物防治 

分 类 号：S763[农业科学—森林保护学]