舞毒蛾幼虫龄数和龄期的划分  

Division of instar number and stage of the spongy moth Lymantria dispar larvae

作　　者：马晓凡 李亚飞 窦烽瑞 艾流卡玛丽·吐木逊 石娟[1,2] Ma Xiaofan;Li Yafei;Dou Fengrui;Ailiukamali Tumuxun;Shi Juan(Beijing Key Laboratory for Forest Pest Control,Beijing Forestry University,Beijing 100083,China;Sino-France Joint Laboratory for Invasive Forest Pests in Eurasia,Beijing Forestry University,Beijing 100083,China)

机构地区：[1]北京林业大学林木有害生物防治北京市重点实验室,北京100083 [2]北京林业大学中法欧亚森林入侵生物联合实验室,北京100083

出　　处：《植物保护学报》2023年第6期1625-1632,共8页Journal of Plant Protection

基　　金：国家自然科学基金(32171794)。

摘　　要：为明确舞毒蛾Lymantria dispar幼虫龄数和龄期划分的形态依据,以室内饲养的来自内蒙古自治区、山西省、辽宁省、贵州省、云南省和四川省的亚洲型舞毒蛾种群以及亚洲型舞毒蛾和欧洲型舞毒蛾的杂交种群作为试验材料,收集每次蜕皮时掉落的头壳并测量其宽度和长度,绘制频数分布图后基于Dyar法则划定幼虫龄数和龄期,并利用Crosby生长法则和线性回归分析进行验证。结果显示,舞毒蛾幼虫头壳宽度和头壳长度的频次分布图可以划分为7个相对集中的区域,1~7龄幼虫的头壳宽度平均值分别为0.55、0.92、1.54、2.42、3.51、4.74和5.88 mm;1~7龄幼虫的头壳长度平均值分别为0.49、0.78、1.21、1.93、2.83、3.89和4.90 mm。幼虫头壳宽度和头壳长度的Crosby指数均小于10%,说明幼虫的头壳宽度和长度均可作为划分龄期的形态指标。在室内饲养过程中,只有小部分雌性舞毒蛾幼虫才能成长至7龄,全部的雄性幼虫和大部分雌性幼虫的龄期为6龄。1~7龄幼虫的众数龄期值分别为10.75、4.99、4.68、6.27、8.24、10.20和4.90 d,整个幼虫众数龄期为50.03 d。To elucidate the morphological basis for determining instar number and stage division in larvae of the spongy moth Lymantria dispar,we utilized populations as experimental materials,including indoor-reared populations of the flighted spongy moth complex from Inner Mongolia Autonomous Region,Shanxi Province,Liaoning Province,Guizhou Province,Yunnan Province,and Sichuan Province,as well as hybrid populations of the flighted spongy moth complex and the spongy moth.During each molting event,shed head capsules were collected,and their width and length were measured to construct frequency distribution graphs.Instar number and stage division were determined using Dyar’s rule,further validated through Crosby’s growth rule,and confirmed using linear regression analysis.The results demonstrated that the frequency distribution graphs of head capsule width and length could be categorized into seven distinct regions with high concentrations.The mean values of head capsule width for instars 1-7 were 0.55,0.92,1.54,2.42,3.51,4.74,and 5.88 mm,respectively.The mean values of head capsule length for instars 1-7 were 0.49,0.78,1.21,1.93,2.83,3.89 and 4.90 mm,respectively.The Crosby index for both head capsule width and length was less than 10%,indicating that both can serve as reliable morphological indicators for larval stage division.The results also revealed that only a small proportion of female spongy moth larvae reached the seventh instar stage;all male larvae,along with most female larvae,attained the sixth instar stage.The modal age values for instars 1-7 were 10.75,4.99,468,627,824,10.20,and 4.90 d,respectively.The entire larval period lasted approximately 50.03 d.

关 键 词：舞毒蛾 幼虫龄期 头壳宽度 头壳长度 形态学特征 

分 类 号：S433.4[农业科学—农业昆虫与害虫防治]