不同品牌胶体金免疫层析试纸条/卡检测牛羊布鲁氏菌病的效果分析  

Analysis on the Effectiveness of Colloidal Gold Immunochromatographic Strips/Cards for Detecting Brucellosis in Cattle and Sheep

作　　者：汪洪冰[1] 郭永祥[1] 张朝阳[1] 黄建龙[1] 张坤 林源[1] Wang Hongbing;Guo Yongxiang;Zhang Chaoyang;Huang Jianlong;Zhang Kun;Lin Yuan(Hunan Center for Animal Disease Prevention and Control,Changsha 410014,Hunan,China)

机构地区：[1]湖南省动物疫病预防控制中心,湖南长沙410014

出　　处：《中国动物检疫》2024年第2期99-102,共4页China Animal Health Inspection

摘　　要：为比较不同品牌胶体金免疫层析试纸条/卡(简称试纸条/卡)检测牛羊布鲁氏菌病的效果,利用抗体布鲁氏菌阴阳性血清,对6种品牌试纸条/卡的准确性及灵敏度进行观察比较;同时按不同试纸条/卡操作方法对经虎红平板凝集试验(RBPT)初筛和竞争酶联免疫吸附试验(cELISA)复核的临床牛羊血清样品进行检测比较。结果显示:不同试纸条/卡对布鲁氏菌企业标准阴阳性血清检出率均为100%,其中2种试纸条/卡敏感性高,对不同稀释倍数阳性血清均可检出;试纸条/卡对临床阴性血清均100%检出,但对临床阳性血清样品的检测结果差异较大,其中对羊阳性血清样品的检出率为5.88%~70.59%,对牛阳性血清样品为50.0%~100.0%。结果表明,不同品牌试纸条/卡在布病检测时存在一定差异。建议使用试纸条/卡进行布鲁氏菌病初筛后,应结合国家相关布病诊断技术进行复检,以确保检测结果的准确性。In order to compare the effectiveness of colloidal gold immunochromatographic strips/cards with different brands for detecting brucellosis in cattle and sheep,6 brands of strips/cards were used to detect antibodies in Brucella negative and positive serum to compare their accuracy and sensitivity;and clinical serum samples that were preliminarily screened using Rose-bengal plate agglutination test(RBPT)and rechecked using compet0itive enzymelinked immunosorbent assay(cELISA)were tested and compared by the strips/cards.The results showed that the detection rates of the strips/cards for enterprise-standard Brucella negative and positive serum samples were all 100%,in which two strips/cards had higher sensitivity and could detect positive serum with different dilution ratios;the strips/cards could detect 100%clinical negative serum samples,but the results for clinical positive serum samples were greatly different,with a detection rate of 5.88%~70.59%for sheep positive serum samples and 50.0%~100.0%for cattle positive serum samples.In conclusion,different brands of strips/cards were with different performance in detecting brucellosis.For the purpose of obtaining accurate test results,it was thus recommended that brucellosis should be reexamined using relevant national diagnostic techniques after it was preliminarily screened by the strips/cards.

关 键 词：胶体金免疫层析法 布鲁氏菌病 效果分析 

分 类 号：S851.3[农业科学—预防兽医学]