山羊口蹄疫、小反刍兽疫疫苗不同免疫剂量同步分点注射免疫效果比较  

Comparison on Immune Effects of Synchronous Multiple-point Injection with Goat FMD and PPR Vaccines at Different Doses

作　　者：高丽艳 杨晓燕 台晓媛 喻永福 张俊雄 李继明 段纲 李本能 李学德 陈关雄 Gao Liyan;Yang Xiaoyan;Tai Xiaoyuan;Yu Yongfu;Zhang Junxiong;Li Jiming;Duan Gang;Li Benneng;Li Xuede;Chen Guanxiong(Center for Animal Disease Prevention and Control of Shilin Yi Autonomous County,Shilin 652200,Yunan,China;Yunnan Xindeshun Animal Husbandry Co.,Ltd.,Shilin 652200,Yunnan,China;College of Veterinary Medicine,Yunnan Agricultural University,Kunming 650201,Yunnan,China;Agricultural and Rural Comprehensive Service Center of Daibu Township,Huize 654216,Yunnan,China)

机构地区：[1]石林彝族自治县动物疫病预防控制中心,云南石林652200 [2]云南鑫德顺畜牧有限公司,云南石林652200 [3]云南农业大学动物医学院,云南昆明650201 [4]会泽县待补镇农业农村综合服务中心,云南会泽654216

出　　处：《中国动物检疫》2024年第2期103-107,共5页China Animal Health Inspection

基　　金：云南省创新与科技型企业培育计划项目(202104BI090017);云南省重大科技专项计划项目(202302AA310020)。

摘　　要：为探究一种适合基层同时开展山羊口蹄疫(FMD)和小反刍兽疫(PPR)疫苗免疫的方法和剂量,选取64只山羊随机分为4组,即常规免疫组(对照组)以及1.0、1.2和1.5头份剂量组(试验组),开展FMD和PPR疫苗常规免疫和不同免疫剂量同步分点注射免疫的效果比较。结果显示:使用上述两种疫苗,采用常规免疫和一次性分点同步注射免疫的方法,以不同免疫剂量同步分点注射接种后,被免疫山羊未出现免疫应激反应;以不同免疫剂量接种后14、28、42、56、70、100和160 d,各组山羊FMD O型、A型以及PPR免疫抗体阳性率差异均无统计学意义(P>0.05)。在接种后14 d,1.2头份剂量组FMD O型免疫抗体阳性率高于1.0和1.5头份剂量组;在接种后100 d,1.5头份剂量组FMD O型免疫抗体阳性率高于其余两剂量组;接种后14 d,对照组山羊FMD A型免疫抗体阳性率为87.5%,其余各组均可达到100%,并维持至接种后100 d。各组山羊PPR抗体阳性率均在接种后14 d达到100%,并持续保持至接种后160 d。结果表明:在试验免疫剂量范围内可开展山羊FMD和PPR疫苗一次性分点同步免疫接种工作,FMD疫苗最佳免疫注射剂量为1.2头份,PPR疫苗为1.0头份,在接种14 d后开展免疫效果监测较好。本研究为基层动物防疫员高效、便利开展山羊FMD和PPR疫苗免疫工作提供了参考。In order to explore a method and dosage for synchronous vaccination for goat foot-and-mouth disease(FMD)and peste des petits ruminants(PPR)at grassroots level,64 goats were randomly selected and divided into 4 groups:one group with routine vaccination(control group)and three experimental groups with synchronous multiple-point vaccination(groups of 1.0,1.2 and 1.5 doses),respectively,to compare the immune effects of different methods for FMD and PPR vaccines.The results showed that no immunological stress reaction was found in the vaccinated goats after the above injections with the two vaccines;and no statistically significant difference was detected among the positive rates of immune antibodies against FMD-O/A virus and PPR virus in each group for 14,28,42,56,70,100 and 160 days after vaccination at different doses(P>0.05).On the 14th day after vaccination,the positive rate of antibodies against FMD-O virus in goats in the group of 1.2 doses was higher than that in the groups of 1.0 and 1.5 doses;on the 100th day after vaccination,the positive rate of antibodies against FMD-O virus in goats in the group of 1.5 doses was higher than that in other two experimental groups;on the 14th day after vaccination,the positive rate of antibodies against FMD-A virus in goats in control group was 87.5%;and that of other groups all reached up to 100%and lasted till on the 100th day.The positive rate of antibodies against PPR virus reached 100%in each group on the 14th day after vaccination and remained till on the 160th day.In conclusion,synchronous multiple-point vaccination with goat FMD and PPR vaccines could be conducted at the experimental doses,specifically,the doses of 1.2 and 1.0 were optimal for the vaccines of FMD and PPR,respectively,and better immune effect should be monitored on the 14th day after vaccination.Grassroots animal health workers were thus provided with a reference to efficiently and conveniently implement vaccination of FMD and PPR vaccines in goats.

关 键 词：口蹄疫 小反刍兽疫 免疫剂量 同步分点注射 

分 类 号：S852.4[农业科学—基础兽医学]