菜缢管蚜的线粒体基因组及蚜亚科的系统发育分析  

Mitochondrial genome of Lipaphis pseudobrassicae(Davis,1914)and phylogenetic analysis of Aphidinae

作　　者：林兴雨 宋南[1] LIN Xingyu;SONG Nan(College of Plant Protection,Henan Agricultural University,Zhengzhou 450046,Henan,China)

机构地区：[1]河南农业大学植物保护学院,河南郑州450046

出　　处：《浙江大学学报（农业与生命科学版）》2024年第1期53-64,共12页Journal of Zhejiang University:Agriculture and Life Sciences

基　　金：国家自然科学基金项目(U1904104)。

摘　　要：菜缢管蚜[Lipaphis pseudobrassicae(Davis,1914)]是一种重要的农业害虫。本研究对菜缢管蚜的线粒体全基因组进行测序、分析,并基于线粒体基因组数据重建蚜亚科(Aphidinae)的系统发育关系。在系统发育分析中,内群包含蚜亚科的31个物种,外群选择绵蚜亚科(Eriosomatinae)中苹果绵蚜(Eriosoma lanigerum)、Paracolopha morrisoni和米倍蚜(Meitanaphis microgallis)3个物种,分别利用最大似然法和贝叶斯法重建蚜亚科的系统发育树。结果表明:菜缢管蚜的线粒体基因组全长16743 bp(GenBank登录号为OP796483),包含37个基因(13个蛋白质编码基因、2个核糖体RNA基因、22个转运RNA基因)和1个非编码控制区。采用最大似然法和贝叶斯法构建的系统发育树均显示蚜族为单系群。在最大似然法分析结果中,长管蚜族为单系群,十字蚜属与短棒蚜属构成姐妹群。然而,在贝叶斯法分析结果中,长管蚜族为非单系群,十字蚜属与短棒蚜属+双尾蚜属亲缘关系较近。本研究为更好地理解长管蚜族、十字蚜属和菜缢管蚜的系统发育关系提供了线粒体基因组数据。Lipaphis pseudobrassicae(Davis,1914)is an important agricultural insect pest.In this study,we sequenced and analyzed the entire mitochondrial genome of L.pseudobrassicae,and reconstructed the phylogenetic relationships of Aphidinae based on the mitochondrial genome data.In phylogenetic analyses,the ingroup included 31 species of Aphidinae,while the outgroup selected three species of Eriosomatinae(Eriosoma lanigerum,Paracolopha morrisoni and Meitanaphis microgallis).The maximum likelihood method and Bayesian method were used to reconstruct the phylogenetic tree of Aphidinae,respectively.The results showed that the mitochondrial genome of L.pseudobrassicae was 16743 bp in length(GenBank accession No.OP796483),which contained 13 protein-coding genes,two ribosomal RNA genes,22 transfer RNA genes and one non-coding control region.The phylogenetic tree constructed by the maximum likelihood method and Bayesian method both showed that the tribe Aphidini was a monophyletic group.The tribe Macrosiphini was recovered as a monophyletic group,and Lipaphis was a sister to Brevicoryne according to the maximum likelihood method.However,the tribe Macrosiphini was recovered as a non-monophyletic group,and Lipaphis was a sister to Brevicoryne+Diuraphis according to the Bayesian method.This study can enhance the understanding of the phylogenetic relationships among Macrosiphini,Lipaphis,and L.pseudobrassicae by utilizing mitochondrial genome data.

关 键 词：半翅目 蚜亚科 菜缢管蚜 线粒体基因组 系统发育 

分 类 号：S436.341.21[农业科学—农业昆虫与害虫防治]