不同玉米自交系对环磺酮的敏感性差异研究  

Sensitivity differences of different maize inbred lines to tembotrione

作　　者：高玥 黄冀楠 许贤[2] 李杰 李秉华[2] 赵铂锤 祁志尊[2] 王贵启[2] 郭建青 刘小民[2] GAO Yue;HUANG Ji’nan;XU Xian;LI Jie;LI Binghua;ZHAO Bochui;QI Zhizun;WANG Guiqi;GUO Jianqing;LIU Xiaomin(College of Agriculture and Forestry Technology,Hebei North University,Zhangjiakou 075000,Hebei Province,China;Institute of Cereal and Oil Crops,Hebei Academy of Agriculture and Forestry Sciences/Key Laboratory of Crop Cultivation Physiology and Green Production of Hebei,Shijiazhuang 050035,China;Shijiazhuang Development and Reform Commission of Hebei Province,Shijiazhuang 050011,China;Field Scientific Observation and Research Station of Forest Pests in Taihang Mountain of Henan,Anyang 455099,Henan Province,China)

机构地区：[1]河北北方学院农林科技学院,河北张家口075000 [2]河北省农林科学院粮油作物研究所,河北省作物栽培生理与绿色生产重点实验室,石家庄050035 [3]河北省石家庄市发展和改革委员会,石家庄050011 [4]河南省太行山林业有害生物野外科学观测研究站,河南安阳455099

出　　处：《农药学学报》2024年第1期132-139,共8页Chinese Journal of Pesticide Science

基　　金：河北省引进留学人员资助项目(C20220355);河北省自然科学基金项目(C2022301006);河南省太行山林业有害生物野外科学观测研究站开放研究课题(THFP202303)。

摘　　要：环磺酮是2021年在中国登记上市的玉米田对羟基苯基丙酮酸双氧化酶(HPPD)抑制剂类除草剂。为明确环磺酮对不同玉米自交系的安全性,以玉米自交系HB05(‘Mo17’)、HB09(‘PH6WC’)、HB39(‘J525’)和HB82(‘’KH8’)为研究对象,进行了环磺酮胁迫对不同玉米自交系幼苗生长及体内生理生化指标的影响研究。结果表明:环磺酮按有效成分120 g/hm^(2)处理,敏感型玉米HB82和HB39表现出叶片失绿白化,直至干枯死亡,严重影响玉米的生长发育;而耐药型玉米HB05和HB09叶片没有出现受害症状,对生长发育无明显影响。环磺酮按有效成分240 g/hm^(2)处理14 d后,对敏感型玉米HB82和HB39株高抑制率分别为64.63%和63.79%,鲜重抑制率分别为70.06%和66.02%。环磺酮按有效成分720 g/hm^(2)处理14 d后,对耐药型玉米HB05和HB09株高抑制率分别为48.61%和38.05%,鲜重抑制率分别为21.93%和43.63%。环磺酮对耐药型玉米HB05和HB09的IC50值分别为223.50和37.42 mg/L,明显高于敏感型玉米HB82和HB39(对的IC50值分别为13.48和16.13 mg/L)。对叶绿素含量测定结果表明:叶绿素a、叶绿素b和叶绿素总含量的变化趋势一致,即随着处理时间的延长,不同玉米自交系体内叶绿素含量呈不同程度的下降趋势,且敏感型玉米下降趋势明显大于耐药型玉米。环磺酮按有效成分120 g/hm^(2)处理7 d后,与0 d相比,敏感型玉米HB82、HB39体内叶绿素总含量较0 d分别下降87.76%和70.55%,而耐药型玉米HB05和HB09分别下降49.86%和33.65%;敏感型玉米HB82和HB39体内类胡萝卜素含量分别下降83.72%和74.02%,耐药型玉米HB05和HB09分别下降40.83%和33.33%。对超氧化物歧化酶(SOD)和过氧化物酶(POD)酶活性的测定结果表明:环磺酮胁迫诱导了不同玉米自交系体内SOD和POD酶活性的增加,呈先上升后下降的趋势,其变化幅度为敏感型玉米高于耐药型玉米。综上所述,不同玉米自交系对环磺酮的敏感性存在�Tembotrione,a 4-hydroxyphenylpyruvate dioxygenase(HPPD)inhibiting herbicide applied in maize fields,was first registered in China in 2021.To clarify the safety of tembotrione on different maize inbred lines,the effects of tembotrione on the growth as well as physiological and biochemical indicators of maize inbred lines HB05(‘Mo17’),HB09(‘PH6WC’),HB39(‘J525’),and HB82(‘KH8’)were studied.The results indicated that under tembotrione stress of 120 g a.i./hm^(2),sensitive maize lines HB82 and HB39 exhibited wilting,chlorosis of leaves,and even death,significantly impairing maize growth.Conversely,the leaves of tolerant maize inbred lines HB05和HB09 showed no signs of injury,and the application of tembotrione had little impact on their growth.The Sensitive varieties HB82 and HB39 exhibited plant height inhibition rates of 64.63%and 63.79%and fresh weight inhibition rates of 70.06%and 66.02%under 240 g a.i./hm^(2)tembotrione treatment for 14 days.In contrast,tolerant varieties HB05 and HB09 showed lower plant height inhibition rates of 48.61%and 38.05%after 720 g a.i./hm^(2)treatment for the same duration,with fresh weight inhibition rates of 21.93%and 43.63%,respectively.The half maximal inhibitory concentration(IC50)values of tembotrione for the root length were 223.50 and 37.42 mg/L for the tolerant HB05 and HB09,whereas the sensitive varieties HB82 and HB39 displayed lower IC50 values,measuring 13.48 mg/L and 16.13 mg/L.Chlorophyll content measurements revealed a declining trend in chlorophyll a,chlorophyll b,and total chlorophyll for both sensitive and resistant maize.Following 7 days of treatment with 120 g a.i./hm^(2)tembotrione,the total chlorophyll content in sensitive maize HB82 and HB39 experienced substantial decreases of 87.76%and 70.55%while the resistant maize HB05 and HB09 showed lower decreases,with reductions of 49.86%and 33.65%,respectively;The carotenoid content determination revealed an 83.72%and 74.02%decrease in sensitive maize varieties HB82 and HB39,respectively,after 7 days of

关 键 词：环磺酮 玉米自交系 敏感性 抑制率 生理生化指标 

分 类 号：S482.4[农业科学—农药学]