实验教学中醋酸纤维素薄膜电泳的条件优化  

Optimization of conditions for cellulose acetate membrane electrophoresis in experimental teaching

作　　者：武丽涛 李梦瑶 宁启兰 吴锋[2] 杨旭东 闫小飞 蒋小英 蒋晓刚 李冬民 WU Litao;LI Mengyao;NING Qilan;WU Feng;YANG Xudong;YAN Xiaofei;JIANG Xiaoying;JIANG Xiaogang;LI Dongmin(Department of Biochemistry and Molecular Biology,School of Basic Medical Sciences,Xi’an Jiaotong University,Xi’an 710061,China;Center of Teaching Experiment for Postgraduate in Medicine,Xi’an Jiaotong University,Xi’an 710061,China)

机构地区：[1]西安交通大学医学部基础医学院生物化学与分子生物学系,陕西西安710061 [2]西安交通大学医学研究生教学实验中心,陕西西安710061

出　　处：《南阳师范学院学报》2024年第1期74-78,共5页Journal of Nanyang Normal University

基　　金：西安交通大学“名课程”《生物化学》第三轮建设立项课程;2021年西安交通大学本科实验实践与创新创业教育教学改革研究专项项目(21SJZX29)。

摘　　要：醋酸纤维素薄膜电泳分离血清蛋白是生物化学实验课中的一项基础实验技术。目前,诸多医学院校基础医学及生物学专业均开设了此项实验。但是由于实验方法的不同,且实验过程中的影响因素较多,学生获得的实验结果也存在多种问题。经过多年实验教学探索,西安交通大学医学部基础医学院生物化学与分子生物系实验教学团队优化了醋酸纤维素薄膜电泳分离血清蛋白的实验方案,增强了实验教学内容的互动性,确保了实验结果的稳定性,使学生更好地掌握了水平电泳分离蛋白质的理论知识并获得了成功的实验验证。Separation of serum proteins by cellulose acetate membrane electrophoresis is a basic experimental technique in biochemistry laboratory classes.At present,many medical schools offer this experiment in basic medicine and biology.However,due to different experimental methods and many influencing factors in the experimental process,the experimental results obtained by students have various problems.After years of experimental teaching exploration,the experimental protocol for the separation of serum proteins by cellulose acetate membrane electrophoresis has been optimized,the interactivity of the experimental teaching content has been enhanced,and the stability of the experimental results has been ensured,which enable the students to better grasp the theoretical knowledge of the horizontal electrophoretic separation of proteins and obtain a successful experimental verification.

关 键 词：生物化学实验教学 醋酸纤维素薄膜电泳 血清蛋白的分离 

分 类 号：Q5-33[生物学—生物化学]