低温弱光下普通丝瓜的生理响应及转录组测序分析  

Physiological Response and Transcriptome Sequencing Analysis of Luffa cylindrica under Low Temperature and Weak Light

作　　者：王炫榛 程玉琦 陈敏氡[1] 刘建汀 花秀凤[3] 叶新如 李永平[1] 温庆放[1] 朱海生[1] WANG Xuan-zhen;CHENG Yu-qi;CHEN Min-dong;LIU Jian-ting;HUA Xiu-feng;YE Xin-ru;LI Yong-ping;WEN Qing-fang;ZHU Hai-sheng(Fujian Key Laboratory of Vegetable Genetics and Breeding/Crops Research Institute,Fujian Academy of Agricultural Sciences/Vegetable Research Center,Fujian Academy of Agricultural Sciences,Fuzhou 350013,China;College of Horticulture,Fujian Agriculture and Forestry University,Fuzhou 350002,China;Fuzhou Institute of Vegetable Science,Fuzhou 350013,China)

机构地区：[1]福建省蔬菜遗传育种重点实验室/福建省农业科学院作物研究所/福建省农业科学院蔬菜研究室,福建福州350013 [2]福建农林大学园艺学院,福建福州350002 [3]福州市蔬菜科学研究所,福建福州350013

出　　处：《江西农业学报》2023年第12期53-63,共11页Acta Agriculturae Jiangxi

基　　金：福建省属公益类科研院所基本科研专项(2022R1031007);福建省自然科学基金项目(2021J01494);国家大宗蔬菜产业技术体系福州综合试验站项目(CARS-23-G-53);福建省农业科学院科技创新团队项目(CXTD2021003-1)。

摘　　要：对普通丝瓜(Luffa cylindrica)品种农福806的幼苗分别进行10℃和5℃低温弱光胁迫处理,通过测定其生理指标、光合参数和叶绿素荧光参数,应用相关性分析、主成分分析和转录组测序等方法研究了普通丝瓜幼苗对低温弱光胁迫的生理响应机制。研究结果表明:在5℃弱光处理期间(0~24 h),普通丝瓜幼苗的过氧化物酶(POD)、过氧化氢酶(CAT)、超氧化物歧化酶(SOD)活性以及丙二醛(MDA)、游离脯氨酸(Pro)含量总体上均呈先上升后下降的变化趋势,可溶性糖含量总体上一直呈上升趋势;在10℃弱光处理期间(0~10 d),丝瓜幼苗的POD活性以及MDA、可溶性糖含量总体上一直呈上升趋势,其他3个指标总体上呈先升后降的变化趋势;丝瓜幼苗的可溶性糖含量与POD、SOD活性呈极显著正相关,与CAT活性呈极显著负相关;Pro含量与MDA含量呈极显著负相关;SOD活性与CAT活性呈极显著负相关,与POD活性呈显著正相关。对6个生理指标进行主成分分析,在5℃下提取出3个主成分,在10℃下提取出2个主成分。转录组测序获得了7.13亿条Clean data,在3个低温处理组丝瓜幼苗的差异表达基因中,上调基因共有4945个,下调基因共有5065个,涉及24种生物学过程,差异表达基因涉及代谢过程、植物激素信号转导、内吞作用和植物─病原互作。总之,低温弱光对普通丝瓜幼苗的光保护系统破坏性较大,光合效率降低会间接引起幼苗脱水萎蔫;SOD活性是丝瓜幼苗响应低温弱光胁迫的主要生理指标;差异表达基因涉及Ca^(2+)转运、海藻糖代谢等生物途径。The seedlings of Luffa cylindrica cultivar‘Nongfu 806’were treated with low temperature and weak light stress at 10℃and 5℃,respectively.Physiological parameters,photosynthetic parameters and chlorophyll fluorescence parameters were determined.Correlation analysis,principal component analysis and transcriptome sequencing were used to study the physiological response mechanism of Luffa cylindrica seedlings to low temperature and low light stress.The results showed that During low light treatment at 5℃(0~24 h),the activities of peroxidase(POD),catalase(CAT),superoxide dismutase(SOD)and the contents of malondialdehyde(MDA)and free proline(Pro)of Luffa cylindrica seedlings increased first and then decreased,while the content of soluble sugar increased in general.During low light treatment at 10℃(0~10 d),POD activity,MDA and soluble sugar content of Luffa cylindrica seedlings showed an increasing trend in general,and the other three indexes showed an increasing trend first and then decreasing trend in general.The content of soluble sugar in luffa seedlings was positively correlated with POD and SOD activities and negatively correlated with CAT activities.Pro content was significantly negatively correlated with MDA content.SOD activity was negatively correlated with CAT activity and positively correlated with POD activity.Principal component analysis was performed on 6 physiological indexes,and 3 principal components were extracted at 5℃and 2 principal components were extracted at 10℃.Transcriptome sequencing obtained 713 million Clean data.Among the differentially expressed genes of luffa luffa seedlings in the three low-temperature treatment groups,there were 4945 up-regulated genes and 5065 down-regulated genes,involving 24 biological processes.Differentially expressed genes were involved in metabolic processes,plant hormone signal transduction,endocytosis and plant-pathogen interaction.In conclusion,low temperature and low light caused great damage to the photoprotection system of Luffa cylindrica se

关 键 词：普通丝瓜 低温 弱光 生理响应 光合特性 转录组测序 

分 类 号：S642.4[农业科学—蔬菜学]