高效液相色谱法测定柴桂解表颗粒中黄芩苷含量的研究  

Determination of Baicalin in Chaiguijiebiao granule by high performance liquid chromatography

作　　者：成程 陈向梅 马健[1] 张沂 Cheng Cheng;Chen Xiangmei;Ma Jian;Zhang Yi(Department of Pharmacy,Medical Security Center,General Hospital of PLA,Beijing 100048,China)

机构地区：[1]解放军总医院医疗保障中心药剂科,北京100048

出　　处：《海军医学杂志》2024年第1期91-94,共4页Journal of Navy Medicine

基　　金：军队医院制剂提升项目(14TG0709)。

摘　　要：目的 建立柴桂解表颗粒中黄芩苷含量测定方法,进一步完善柴桂解表颗粒的质量标准。方法 超声处理柴桂解表颗粒样品,制备供试品溶液。采用高效液相色谱法进行专属性试验、线性关系考察、精密度试验、重复性试验、加样回收率试验等方法学考察,在方法学考察合格的基础上,对3批柴桂解表颗粒样品中的黄芩苷含量进行测定。结果 3批样品中的黄芩苷含量分别为4.07、4.11、4.40 mg/g,初步制定柴桂解表颗粒质量标准中黄芩苷含量为4.0~4.5 mg/g。结论 高效液相色谱法操作简便,结果准确可靠,可作为柴桂解表颗粒中黄芩苷含量的测定方法。Objective To establish a method for the determination of baicalin in Chaiguijiebiao granule by high performance liquid chromatography(HPLC)and further improve the quality standards of Chaiguijiebiao granule.Methods The sample of Chaiguijiebiao granule was treated by ultrasound to prepare the test product solution.Specificity test,linear relationship test,precision test,repeatability test,and sample recovery test were investigated by HPLC.On the basis of qualified methodology,the content of baicalin in 3 batches of Chaiguijiebiao granule was determined.Results The concentration of baicalin in the 3 batches of samples was 4.07 mg/g,4.11 mg/g and 4.40 mg/g,respectively.Preliminary quality standard of Chaiguijiebiao granule was established,with baicalin content ranging from 4.0 to 4.5 mg/g.Conclusion The HPLC method is simple,accurate and reliable,and can be used for the determination of baicalin in Chaiguijiebiao granule.

关 键 词：柴桂解表颗粒 黄芩苷 高效液相色谱法 含量测定 

分 类 号：R917[医药卫生—药物分析学]