水稻SBP基因家族的全基因组研究  

Genome-Wide Study of the Rice SBP Gene Family

作　　者：庄可卿 杜娟[3] 翁雅萍 许雅琴 熊娟[1] 邓志龙 刘昊霖 刘豫 宋惠 李华[1] 杨婷 宁德正 刘利莎 夏海林[1] 卫海滨[2] 张琳[1] 胡颂平[1] ZHUANG Keqing;DU Juan;WENG Yaping;XU Yaqin;XIONG Juan;DENG Zhilong;LIU Haolin;LIU Yu;SONG Hui;LI Hua;YANG Ting;NING Dezheng;LIU Lisha;XIA Hailin;WEI Haibin;ZHANG Lin;HU Songping(Institute of Plant Functional Genes and Tissue Culture Technology,College of Bioscience andBioengineering,Jiangxi Agricultural University,Nanchang 330045,China;Shanghai Agrobiological GeneCenter,Shanghai 201106,China;Lushan Botanical Garden,Chinese Academy of Sciences,Jiujiang,Jiangxi332900,China)

机构地区：[1]江西农业大学植物功能基因与组织培养技术研究中心,江西南昌330045 [2]上海市农业生物基因中心,上海201106 [3]中国科学院庐山植物园,江西九江332900

出　　处：《江西农业大学学报》2023年第6期1315-1323,共9页Acta Agriculturae Universitatis Jiangxiensis

基　　金：国家自然科学基金项目(31971926);江西省自然科学基金联合资助重点项目(20202ACBL205007)

摘　　要：【目的】水资源紧缺严重威胁我国的粮食安全,优化水稻的节水抗旱性状是推广旱作农业节水的最为经济有效的方法。Squamosa promoter binding protein(SBP)基因家族是一类具有重要应用价值的植物特异性转录因子,它们可以影响植物的株型、产量和抗逆性,为节水抗旱育种提供了重要资源。然而,SBP家族成员在水稻发育和胁迫响应中的功能目前还未全部鉴定,调控抗逆性研究也未进行。【方法】利用SBP结构域一致性序列搜索水稻基因组,再进行基因结构和系统发育分析、共线性分析及基因表达模式分析。【结果】鉴定出19个SBP-like(OsSPL)基因,分布于10条染色体上,编码蛋白序列长度为217~1141 aa。基因结构和系统发育分析表明水稻OsSPL基因可分成4组。共线性分析表明,水稻基因组内OsSPL-4和OsSPL-5形成1个串联重复基因簇,11个OsSPL,形成7个跨染色体分布的重复基因对。水稻和拟南芥SPL形成5个同源基因对,与玉米形成45个同源基因对。Evorepro数据库中OsSPL基因表达模式分析表明水稻叶片冷胁迫下OsSPL-1和OsSPL-3表达下调,OsSPL-2和OsSPL-9表达上调;干旱胁迫下OsSPL-1、OsSPL-4和OsSPL-8转录水平下调;OsSPL-2和OsSPL-14(IPA1)转录水平上调。【结论】OsSPL基因在水稻基因组中广泛分布,并形成多个跨染色体基因对;同时在逆境如寒冷、干旱等胁迫下可做出较大反应,从而表现可调控水稻的耐逆、抗逆性。研究结果可为节水抗旱稻育种改良和机理解析提供理论依据。[Objective]Since the shortage of water resources seriously threatens China’s food security,op-timizing the water-saving and drought resistance characteristics of rice is the most economical and effective method to promote water-saving in dryland agriculture.The plant-specific transcription factor squamosa pro-moter binding protein(SBP)affects plant type,yield and stress resistance,which provides important support for water-saving and drought-resistant breeding.However,the functions of SBP family members in rice develop-ment and stress response have not been fully identified,and the regulation of stress resistance has not been stud-ied.[Method]This study utilized SBP domain consistent sequences to search for the rice genome,followed by gene structure and phylogenetic analysis,Co-linear analysis,and gene expression pattern analysis.[Result]19 SBP-LIKE(OsSPL)genes were identified on 10 chromosomes,and the encoding length of amino acid residues ranged from 217 to 1141.Gene structure and phylogenetic analysis suggested that rice OsSPL gene could be di-vided into four groups.Co-linear analysis showed that OsSPL-4 and OsSPL-5 formed a tandem repeat gene cluster within the rice genome,and 11 OsSPLs formed 7 cross chromosomal duplicate gene pairs.5 homologous gene pairs were formed between rice and Arabidopsis SPL,and 45 homologous gene pairs were formed between rice and maize.Furthermore,the analysis of OsSPL gene expression patterns in the Evorepro database showed that under cold stress,the expression of OsSPL-1 and OsSPL-3 in rice leaves was down regulated,while the ex-pression of OsSPL-2 and OsSPL-9 was up regulated;Under drought stress,the transcription levels of OsSPL-1,OsSPL-4 and OsSPL-8 were down-regulated;The transcription levels of OsSPL-2 and OsSPL-14(IPA1)were up-regulated.[Conclusion]The OsSPL gene is widely distributed in the rice genome and forms multiple gene pairs cross chromosome;Under the stresses such as cold and drought,it can make significant responses and thus demonstrate the ability to regulate

关 键 词：水稻 SBP-box SPL 基因组共线性 表达分析 抗逆性 

分 类 号：S511.032[农业科学—作物学]