不同碳源下共存细菌对铜绿微囊藻生长的影响  

Effects of coexisting bacteria on the growth of Microcystis aeruginosa under different carbon sources

作　　者：孙华龙 陈国炜[1] 吴雪菲 方志鹏 贾伟 刘丽[1] SUN Hualong;CHEN Guowei;WU Xuefei;FANG Zhipeng;JIA Wei;LIU Li(School of Civil and Hydraulic Engineering,Hefei University of Technology,Hefei 230009,China)

机构地区：[1]合肥工业大学土木与水利工程学院,安徽合肥230009

出　　处：《合肥工业大学学报（自然科学版）》2024年第2期154-160,232,共8页Journal of Hefei University of Technology：Natural Science

基　　金：国家自然科学基金资助项目(42277298)。

摘　　要：铜绿微囊藻(Microcystis aeruginosa)可通过二氧化碳浓缩机制(carbon concentrating mechanism,CCM)利用水环境中的无机碳进行光合固碳来促进自身生长,是引发淡水蓝藻水华的优势种。自然水体生态系统中菌藻共生体系是其重要组成部分,对藻华起到重要调控作用,但有关不同碳源条件下共生细菌对蓝藻藻华的影响仍不清楚。文章以铜绿微囊藻为模式蓝藻,探究在不同碳源组合下共存细菌对其生长的影响。在总碳(total carbon,TC)质量浓度为25 mg/L的无机碳源和有机碳源组合,环境初始菌藻比为1∶2时,铜绿微囊藻的最大生物量达到最大值5.0×10^(7)个/mL,是纯藻对照组最大生物量的2.05倍;在加入25 mg/L的有机碳源,初始菌藻比为5∶1时,培养周期内可预测的最大生物量达到最小,为1.5×10^(7)个/mL,仅为纯藻对照组最大生物量的0.76倍。结果表明:不同碳源下初始细菌的出现会极大程度地影响铜绿微囊藻的生长,其中,在无机碳源和混合碳源的营养条件下,初始细菌显著加快了铜绿微囊藻的生长繁殖。Microcystis aeruginosa can utilize the inorganic carbon in water environment for photosynthesis and carbon fixation through carbon concentrating mechanism(CCM)to promote its growth,which is the dominant species that causes freshwater cyanobacteria bloom.The algal-bacterial symbiosis system is an important part of natural water systems,which plays an important role in regulating algal blooms,while the system under different carbon source combinations adds many unknowns to the growth and reproduction of algae.In this paper,Microcystis aeruginosa was selected as the research object to explore the effects of exogenous carbon source on the growth of Microcystis aeruginosa under different initial ratios of bacteria to algae.When the concentration of the total carbon(TC)was 25 mg/L,the combination of inorganic carbon source and organic carbon source was added,and the initial ratio of bacteria to algae in the environment was 1∶2,the predicted maximum biomass of Microcystis aeruginosa in the culture cycle reached 5.0×10^(7) cell/mL,which was 2.05 times higher than that of the pure algae control group.When 25 mg/L organic carbon source was added and the initial ratio of bacteria to algae was 5∶1,the predicted maximum biomass in the culture cycle reached the minimum,which was 1.5×10^(7) cell/mL,which was only 0.76 times that of the pure algae control group.The results showed that the addition of initial bacteria under different carbon sources would greatly affect the growth of Microcystis aeruginosa.Among them,under the nutritional conditions of inorganic carbon source and mixed carbon source,the addition of initial bacteria would greatly accelerate the growth and reproduction of Microcystis aeruginosa.

关 键 词：碳源 共存细菌 微藻生长 菌藻比 

分 类 号：X52[环境科学与工程—环境工程]