干燥方式对绿碎茶多酚提取物体外抗氧化和抗糖尿病及乙酰胆碱酯酶抑制活性的影响  

Effects of different drying methods on in vitro antioxidant,anti-diabetic,and acetylcholinesterase inhibitory activities of polyphenol extracts from broken green tea

作　　者：张倜培 李青 包鸿慧 沈正兴 石娟 唐前勇[2] 程一方[2] 周睿 ZHANG Tipei;LI Qing;BAO Honghui;SHEN Zhengxin;SHI Juan;TANG Qianyong;CHENG Yifang;ZHOU Rui(International Scientific and Technological Cooperation Base for Research and Development of Traditional Medicine and Food Homology,Hubei University of Arts and Science,Xiangyang 441053,China;Xiangyang Academy of Agricultural Science,Xiangyang 441607,China)

机构地区：[1]湖北文理学院,传统药食同源产品研究与开发国际科技合作基地,湖北襄阳441053 [2]襄阳市农业科学院,湖北襄阳441607

出　　处：《食品与发酵工业》2024年第4期77-84,共8页Food and Fermentation Industries

基　　金：国家自然科学基金(31201333);国家外国专家项目(G2022027014L);湖北省引进外国人才和智力项目(2022EJD038);教育部产学合作协同育人项目(220600650213503);中韩青年科学家交流计划项目(202201103);国家级大学生创新创业训练计划项目(202210519006);湖北文理学院大学生创新创业训练计划项目(X202210519157);襄阳市研究与开发项目(2022ABA006953)。

摘　　要：研究不同干燥方式处理的绿碎茶多酚提取物体外抗氧化和抗糖尿病活性及对乙酰胆碱酯酶抑制作用。以绿碎茶为试验材料,采用蒸干结合真空干燥与真空冷冻干燥2种干燥工艺对绿碎茶乙酸乙酯萃取相进行干燥处理,比较研究了2种提取物的色泽,DPPH自由基、ABTS阳离子自由基、羟自由基(·OH)清除能力和铁离子还原能力以及α-淀粉酶、α-葡萄糖苷酶、乙酰胆碱酯酶的抑制能力和抗糖基化能力。结果表明,真空冷冻干燥绿碎茶多酚提取物(BGTE-FD)较蒸干结合真空干燥绿碎茶多酚提取物(BGTE-SVD)颜色相对偏黄,具有较强的DPPH自由基、ABTS阳离子自由基、·OH清除能力和铁离子还原能力,其IC_(50)值分别为2.64、5.17、247.01、27.73 mg/L。同时,BGTE-FD具有较高的α-淀粉酶、乙酰胆碱酯酶和α-葡萄糖苷酶抑制作用,IC_(50)值分别为85.84、416.45、4.38 mg/L,且其对α-葡萄糖苷酶的抑制能力显著强于阿卡波糖(IC_(50)值为929.59 mg/L)。此外,BGTE-FD和BGTE-SVD对晚期糖基化终产物抑制率分别高达65.28%和63.66%,略低于氨基胍(71.81%)。因此,真空冷冻干燥绿碎茶多酚提取物具有较强的体外抗氧化和抗糖尿病活性及一定的乙酰胆碱酯酶抑制能力,绿碎茶可作为降血糖和抗痴呆产品的潜在资源。The in vitro antioxidant,anti-diabetic,and acetylcholinesterase inhibitory activities of polyphenol extracts from broken green tea under different drying treatments were investigated in this study.Two different drying processes,including steam drying combined with vacuum drying and vacuum-freeze drying,were used to dry the ethyl acetate extraction phase of broken green tea,the color values,scavenging abilities of DPPH free radicals,ABTS cationic radicals,and·OH,FRAP antioxidant capacities,and the inhibitory activities againstα-amylase,α-glucosidase,and acetylcholine esterase,as well as anti-glycosylation activities of two extracts,were comparatively evaluated.Results showed that the vacuum-freeze drying polyphenol extract from broken green tea(BGTE-FD)displayed a deeper yellow color and stronger scavenging abilities of DPPH free radicals,ABTS cationic radicals,and·OH,and FRAP antioxidant capacities with the IC_(50)values of 2.64 mg/L,5.17 mg/L,247.01 mg/L,and 27.73 mg/L,respectively,and meanwhile exhibited higher inhibitory activities againstα-amylase,acetylcholine esterase,andα-glucosidase with the IC_(50)values of 85.84 mg/L,416.45 mg/L,and 4.38 mg/L,respectively,compared with the steam drying combined with vacuum drying polyphenol extract from broken green tea(BGTE-SVD).In addition,BGTE-FD showed strongerα-glucosidase inhibition than that of acarbose(IC_(50)=929.59 mg/L).Moreover,the inhibition rates of BGTE-FD and BGTE-SVD on advanced glycosylation end products were 65.28%and 63.66%,respectively,slightly lower than that of aminoguanidine(71.81%).Therefore,the polyphenol extracted from broken green tea by vacuum-freeze drying method has strong in vitro antioxidant and anti-diabetic activity and certain inhibitory activity against acetylcholinesterase,and the broken green tea can be used as a potential natural resource for the development of hypoglycemic and anti-dementia products.

关 键 词：绿碎茶 多酚提取物 抗氧化活性 抗糖尿病活性 乙酰胆碱酯酶抑制活性 

分 类 号：TS272.51[农业科学—茶叶生产加工]