蛋清多肽-铁螯合物的制备、表征及对Caco-2细胞的促增殖作用  

Preparation,characterization,and proliferation by Caco-2 cells of egg white polypeptide-iron chelate

作　　者：王晴晴 蔡为荣[1] 汪玉玲 卓允允 黄伟[1] 曹宇凡 WANG Qingqing;CAI Weirong;WANG Yuling;ZHUO Yunyun;HUANG Wei;CAO Yufan(School of Biological and Food Engineering,Anhui Polytechnic University,Wuhu 241000,China)

机构地区：[1]安徽工程大学生物与食品工程学院,安徽芜湖241000

出　　处：《食品与发酵工业》2024年第4期197-203,共7页Food and Fermentation Industries

基　　金：安徽省重点研发项目(202004a06020022)。

摘　　要：为开发人体易吸收的铁补充剂,提高咸蛋清的附加值,该研究以铁螯合能力和水解度(degree of hydrolysis,DH)为指标,优化酶解条件,通过超滤和Sephadex G-15凝胶柱分离纯化铁螯合能力高的蛋清多肽。采用扫描电镜、能谱、红外光谱和X射线衍射(X-ray diffraction,XRD)对蛋清多肽-铁螯合物结构进行表征。同时探究其对Caco-2细胞活力的影响。结果表明,最佳酶解条件为底物质量分数4%、酶添加量10%、pH 8、温度50℃时酶解8 h,所得产物的铁螯合能力为95.27%。蛋清粗肽经超滤和Sephadex G-15分离纯化得到铁螯合能力最高的组分F4-4。扫描电镜、能谱、红外光谱和XRD结果表明蛋清多肽和Fe2+形成多肽-铁螯合物。细胞实验中,与蛋清多肽和硫酸亚铁相比,蛋清多肽-铁螯合物的安全性高,且有一定的促增殖作用。This study aimed to produce iron supplements that can be easily absorbed by humans and increase the added value of salted egg white.The optimum enzymatic hydrolysis conditions were obtained based on the iron chelating capacity and degree of hydrolysis,and the polypeptides with high iron chelating capacity were separated and purified by ultrafiltration and Sephadex G-15 gel column.The structure of egg white polypeptide-iron chelate was characterized by scanning electron microscopy,energy spectrum,infrared spectroscopy,and X-ray diffraction(XRD).At the same time,this study also aimed to explore its effect on the cell activity of Caco-2.Results showed that the optimal conditions of enzymatic hydrolysis were that substrate concentration was 4%,enzyme addition was 10%,pH was 8,temperature was 50℃,and enzymatic hydrolysis time was 8 h.The iron chelating capacity of the product was 95.27%.F4-4 was the highest iron chelating capacity component,which was obtained from egg white polypeptide by ultrafiltration and Sephadex G-15.The results of the scanning electron microscope,energy spectrum,infrared spectrum,and XRD showed that the egg white polypeptide formed a chelate with Fe 2+.In the cell experiment,compared with egg white polypeptide and ferrous sulfate,the egg white polypeptide-iron was safer and could have a certain role in promoting cell proliferation.

关 键 词：咸蛋清 铁结合肽 分离纯化 结构表征 细胞增殖 

分 类 号：TS253.1[轻工技术与工程—农产品加工及贮藏工程]