链脲佐菌素与特殊膳食诱导的糖尿病动物模型的建立及比较实验  

Establishment and Comparison of Animal Models of Diabetes Mellitus Induced by Streptozocin and Special Diet

作　　者：卢丽 李哲轩 刘子维[1] LU Li;LI Zhe-xuan;LIU Zi-wei(School of Chemical Engineering and Pharmacy,Wuhan University of Engineering,Wuhan 430205,Hubei,China)

机构地区：[1]武汉工程大学化工与制药学院,湖北武汉430205

出　　处：《医学信息》2024年第3期125-127,132,共4页Journal of Medical Information

摘　　要：目的比较两种不同糖尿病动物模型的建立及作用。方法将40只雄鼠ICR小鼠随机分为正常对照组和不同剂量链脲佐菌素组(120、130、140 mg/kg)。健康清洁级雄性小鼠20只,分为普食对照组和高脂饮食组,比较正常小鼠与不同剂量STZ组血糖值,普食组与高脂组喂养第1、4、8、12、16周体重,分析高脂饮食喂养对糖尿病模型动物糖耐量的影响。结果STZ高剂量组空腹血糖水平与正常对照组比较,差异有统计学意义(P<0.05)。高脂组高脂饮食喂养第4、8、12周体重高于普食组,差异有统计学意义(P<0.05)。第16周体重高于普食组,但差异无统计学意义(P>0.05)。第16周的喂养后高脂组给糖后30、60、120 min血糖值高于普食组,差异有统计学意义(P<0.05)。结论链脲佐菌素诱导的糖尿病动物模型可有效模拟1型糖尿病病程及发病机理,而特殊膳食诱导建立2型糖尿病动物模型适用于对降糖药物的临床前筛选实验。Objective To compare the establishment and effects of two different animal models of diabetes mellitus.Methods Forty male ICR mice were randomly divided into normal control group and different doses of streptozotocin groups(120,130 and 140 mg/kg).Twenty healthy male mice of clean grade were divided into normal diet control group and high-fat diet group.The blood glucose levels of normal mice and different doses of STZ groups were compared.The body weights of normal diet group and high-fat diet group were fed for 1,4,8,12 and 16 weeks.The effect of high-fat diet on glucose tolerance of diabetic mellitus model animals was analyzed.Results There was a statistically significant difference in fasting blood glucose levels between the high-dose STZ group and the normal control group(P<0.05).The body weight of the high-fat diet group was higher than that of the normal diet group on the 4th,8th and 12th weeks of high-fat diet feeding,and the difference was statistically significant(P<0.05).The body weight of the 16th week was higher than that of the normal diet group,but the difference was not statistically significant(P>0.05).After 16 weeks of feeding,the blood glucose levels in the high-fat group were higher than those in the normal diet group at 30,60,and 120 min after glucose administration,and the difference was statistically significant(P<0.05).Conclusion The animal model of diabetes mellitus induced by streptozotocin can effectively simulate the course and pathogenesis of type 1 diabetes mellitus,while the animal model of type 2 diabetes mellitus induced by special diet is suitable for preclinical screening of hypoglycemic drugs.

关 键 词：链脲佐菌素 糖尿病 血糖 高脂饮食诱导 动物模型 

分 类 号：R587.1[医药卫生—内分泌] R-332[医药卫生—内科学]