大黄酚调控Wnt/β-catenin通路对胶质瘤U87细胞增殖、凋亡、侵袭能力及EMT的影响  

Effects of Chrysophanol Regulating Wnt/β-catenin Pathway on Proliferation,Apoptosis,Invasion Ability and EMT of Glioma U87 Cells

作　　者：韩庆亮 张慧[1] 郑慧军[2] HAN Qingliang;ZHANG Hui;ZHENG Huijun(Henan University of Chinese Medicine,Zhengzhou Henan 450046,China;Henan Province Hospital of Traditional Chinese Medicine,Zhengzhou Henan 450002,China)

机构地区：[1]河南中医药大学,河南郑州450046 [2]河南省中医院,河南郑州450002

出　　处：《新中医》2023年第24期112-118,共7页New Chinese Medicine

基　　金：河南省中医药科学研究专项课题(2022ZY2014);河南省中医药科学研究专项课题(2019ZY2052)。

摘　　要：目的:探讨大黄酚调控Wnt/β-catenin信号通路抑制胶质瘤细胞的作用机制。方法:体外培养人脑胶质瘤细胞U87,用不同浓度(0、50、100、200μmol/L)的大黄酚处理后,采用CCK-8法检测U87细胞增殖能力;划痕法检测细胞迁移能力;Transwell法检测细胞侵袭能力;流式细胞术检测细胞凋亡;蛋白质印迹法检测凋亡相关蛋白Bax、Bcl-2以及Wnt通路上皮细胞-间充质转化(EMT)相关蛋白β-catenin、E-cadherin、vimentin、MMP-9蛋白表达。结果:大黄酚能抑制U87细胞增殖、迁移及侵袭,诱导凋亡(P<0.05),下调Bcl-2、β-catenin、vimentin与MMP-9的表达水平,上调Bax与E-cadherin的表达水平(P<0.05),并呈浓度依赖关系。结论:大黄酚能抑制胶质瘤细胞U87增殖、迁移及侵袭能力,其机制可能与抑制Wnt/β-catenin信号通路有关。Objective:To investigate the mechanism of chrysophanol regulating the Wnt/β-catenin signaling pathway to inhibit glioma cells.Methods:Human glioma cells U87 were cultured in vitro and treated with different concentrations of chrysophanol(0,50,100,200 μmol/L).The proliferation capacity of U87 cells was detected by CCK-8 method.Cell migration ability was detected by scratch method.Cell invasion ability was detected by Transwell method.Flow cytometry was used to detect apoptosis.Western Blot was used to detect the expressions of apoptosis-related proteins Bax,Bcl-2 and β-catenin,Ecadherin,vimentin and MMP-9 proteins related to Epithelial-mesenchymal transition(EMT) in Wnt pathway.Results:Rhein could inhibit proliferation,migration and invasion of U87 cells,induce apoptosis(P<0.05),down-regulate the expression levels of Bcl-2,β-catenin,vimentin and MMP-9,and upregulate the expression levels of Bax and E-cadherin(P<0.05),in a dose-dependent relationship.Conclusion:Chrysophanol can inhibit the proliferation,migration and invasion of glioma cells U87,and its mechanism may be related to the inhibition of Wnt/β-catenin signaling pathway.

关 键 词：大黄酚 胶质瘤细胞U87 WNT/Β-CATENIN信号通路 上皮细胞-间充质转化 

分 类 号：R285[医药卫生—中药学]