电针抑制AT1介导JAK/STAT通路治疗高血压左室肥厚大鼠实验研究  

Effects of Electroacupuncture on Hypertensive Left Ventricular Hypertrophy by Inhibiting AT1-Mediated JAK/STAT Pathway in Rats

作　　者：康永清 罗勇 唐新桥 吴双华 王婷 KANG Yongqing;LUO Yong;TANG Xinqiao;WU Shuanghua;WANG Ting(Zhuzhou Central Hospital,Zhuzhou Hunan 412007,China)

机构地区：[1]株洲市中心医院,湖南株洲412007

出　　处：《新中医》2023年第24期129-133,共5页New Chinese Medicine

基　　金：湖南省中医药科研计划项目(2021130);株洲市中心医院院级课题(202237)。

摘　　要：目的:探讨电针治疗自发性高血压左室肥厚大鼠的信号传导机制。方法:自发性高血压大鼠(SHR)随机分为高血压左室肥厚组、电针穴位组、电针非穴组、AT1-R siRNA组及STAT siRNA组,以血压正常Wistar-Kyoto大鼠作为对照组,每组20只。观察大鼠治疗前后的血压、心脏结构改变、心肌纤维化情况、血管紧张素Ⅱ(Ang Ⅱ)浓度、血管紧张素受体1 (AT1) mRNA、Janus激酶及转录的信号转导子及激活子(JAK/STAT)蛋白表达的情况。结果:电针穴位组治疗后大鼠收缩压下降,而高血压左室肥厚组、电针非穴组、AT1-R siRNA组和STAT siRNA组大鼠治疗后收缩压无明显下降,与电针穴位组比较,差异均有统计学意义(P<0.05)。电针穴位组大鼠的左心室质量指数(LVMI)小于高血压左室肥厚组、电针非穴组、AT1-R si RNA组和STAT siRNA组,差异有统计学意义(P<0.05)。电针穴位组大鼠的左室短轴缩短率(LVFS)高于高血压左室肥厚组、电针非穴组、AT1-R siRNA组和STAT siRNA组,差异有统计学意义(P<0.05)。HE染色切片可观察到:电针穴位组大鼠的心脏较高血压左室肥厚组大鼠心脏体积和心肌细胞横截面积缩小。电针穴位组大鼠心肌组织Ang Ⅱ水平、血清血清型前胶原(PCⅢ)、层粘连蛋白(LN)、透明质酸(HA)水平均低于高血压左室肥厚组,差异均有统计学意义(P<0.05)。电针穴位组大鼠心肌组织AT1mRNA水平低于高血压左室肥厚组,差异有统计学意义(P<0.05)。高血压左室肥厚组、电针非穴组、AT1-R siRNA组JAK1、STAT3高表达,而电针穴位组JAK1、STAT3表达减少,两者差异均有统计学意义(P<0.05)。结论:电针治疗可能是通过抑制AT1介导的JAK/STAT信号通路,抑制相关细胞因子和蛋白的表达,达到逆转大鼠左室肥厚的作用。Objective:To investigate the signal transduction mechanism of electroacupuncture in spontaneously hypertensive left ventricular hypertrophy rats.Methods:Spontaneous hypertensive rats(SHR) were randomly divided into hypertensive left ventricular hypertrophy group,electroacupuncture point group,electroacupuncture non-point group,AT1-R siRNA group and STAT siRNA group.Wistar-Kyoto rats with normal blood pressure were used as control group,with 20 rats in each group.Blood pressure,cardiac structure changes,myocardial fibrosis,angiotensin Ⅱ(Ang Ⅱ) concentration,angiotensin receptor1(AT1) mRNA,Janus kinase and expression of transcriptional signal transducers and activators(JAK/STAT)protein were observed before and after treatment.Results:The systolic blood pressure of rats in the electroacupuncture point group decreased after treatment,but the systolic blood pressure of rats in the left ventricular hypertrophy group,the non-point group of electroacupuncture,the AT1-R siRNA group and the STAT siRNA group did not decrease significantly after treatment,and the differences were statistically significant(P<0.05).The left ventricular mass index(LVMI) in electroacupuncture point group was lower than that in hypertensive left ventricular hypertrophy group,electroacupuncture non-point group,AT1-R siRNA group and STAT siRNA group,and the difference was statistically significant(P<0.05).The left ventricular short axis shortening rate(LVFS) in electroacupuncture point group was higher than that in hypertensive left ventricular hypertrophy group,electroacupuncture non-point group,AT1-R siRNA group and STAT siRNA group,and the difference was statistically significant(P<0.05).HE staining showed that the heart volume and cross-sectional area of myocardium of rats in the electroacupuncture point group were smaller than those in the hypertensive left ventricular hypertrophy group.The levels of Ang Ⅱ in myocardial tissue,serum procollagen(PCⅢ),laminin(LN) and hyaluronic acid(HA) in electroacupuncture point group were lower than t

关 键 词：高血压 左室肥厚 电针 血管紧张素受体1 Janus激酶及转录的信号转导子及激活子 

分 类 号：R245.3[医药卫生—针灸推拿学]