蛇床子素对急性胰腺炎模型大鼠的治疗作用  

Therapeutic effects of osthol on acute pancreatitis model rats

作　　者：高海运 申立静 刘海容[3] 王雪珍[4] 张翼飞 李佳 GAO Hai-yun;SHEN Li-jing;LIU Hai-rong;WANG Xue-zhen;ZHANG Yi-fei;LI Ji(Emergency,Hebei Provincial Hospital of Traditional Chinese Medicine,Shijiazhuang 050000,Hebei Province;Department of Intensive Care Medicine,Hebei Provincial Hospital of Traditional Chinese Medicine,Shijiazhuang 050000,Hebei Province;Department of Pharmacy,Hebei Provincial Hospital of Traditional Chinese Medicine,Shijiazhuang 050000,Hebei Province;Outpatient Department,Hebei Provincial Hospital of Traditional Chinese Medicine,Shijiazhuang 050000,Hebei Province)

机构地区：[1]河北省中医院急诊科,河北石家庄050000 [2]河北省中医院重症医学科,河北石家庄050000 [3]河北省中医院药学部,河北石家庄050000 [4]河北省中医院门诊部,河北石家庄050000

出　　处：《中国临床药理学杂志》2024年第3期403-407,共5页The Chinese Journal of Clinical Pharmacology

基　　金：河北省中医药管理局科研计划基金资助项目(2020044)。

摘　　要：目的 探究蛇床子素对急性胰腺炎(AP)大鼠的治疗作用及在该过程中对磷酸肌醇3-激酶(PI3K)/蛋白激酶B(Akt)信号通路的调节机制。方法 将SPF级SD雄性大鼠随机分为对照组、模型组(50μg·kg^(-1)雨蛙素+10 mg·kg^(-1) LPS)、阳性对照组(2 mg·kg^(-1)地塞米松)、低剂量实验组(20 mg·kg^(-1)蛇床子素)、高剂量实验组(40 mg·kg^(-1)蛇床子素)、高剂量+740Y-P组(40 mg·kg^(-1)蛇床子素+2 mg·kg^(-1) PI3K激活剂740Y-P),每组15只。末次给药结束24 h后,用全自动生化分析仪检测大鼠血清中淀粉酶和脂肪酶活性;酶联免疫吸附试验(ELISA)法检测大鼠血清中炎性因子肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)的水平以及胰腺组织中丙二醛(MDA)含量和超氧化物歧化酶(SOD)活性,用苏木精-伊红(HE)染色观察大鼠胰腺组织的病理变化并进行病理损伤评分,用蛋白质印迹法检测大鼠胰腺组织中PI3K/Akt通路相关蛋白的表达水平。结果 对照组、模型组、阳性对照组、高剂量实验组和高剂量+740Y-P组的血清淀粉酶活性分别为(135.67±12.89)、(1 027.84±32.16)、(174.31±15.27)、(186.70±17.39)和(835.92±28.78)U·mL^(-1),TNF-α的含量分别为(35.69±3.10)、(223.54±15.23)、(48.76±4.25)、(52.31±4.68)和(208.46±13.65)pg·mL^(-1),MDA的含量分别为(2.15±0.14)、(6.37±0.42)、(2.78±0.17)、(2.81±0.15)和(5.96±0.36)nmol·mg^(-1),组织病理损伤评分分别为(1.12±0.07)、(10.23±0.38)、(3.14±0.21)、(3.25±0.23)和(9.68±0.40)分,p-PI3K/PI3K的比值分别为0.82±0.05、1.96±0.15、1.07±0.06、1.10±0.07和1.69±0.14。模型组的上述指标与对照组比较,阳性对照组、高剂量实验组的上述指标与模型组比较,高剂量+740Y-P组的上述指标与高剂量实验组比较,在统计学上差异均有统计学意义(均P<0.05)。结论 蛇床子素可以对AP发挥治疗作用,其作用机制可能与抑制PI3K/Akt信号通路有关。Objective To investigate the therapeutic effect of cnithol on acute pancreatitis(AP) rats and its regulatory mechanism on phosphoinositol 3-kinase(PI3K)/protein kinase B(Akt) signaling pathway.Methods SPF-grade SD male rats were randomly divided into control group,model group(50 μg·kg^(-1) hyranin +10 mg·kg^(-1) LPS),positive control group(2 mg·kg^(-1) dexamethasone),experimental-L group(20 mg·kg^(-1) osthol) and experimental-H group(40 mg·kg^(-1) osthol),experimental-H+740Y-P group(40 mg·kg^(-1) osthol +2 mg·kg^(-1) PI3K activator 740Y-P),15 mice in each group.The activities of amylase and lipase in serum of rats were detected by automatic biochemical analyzer 24 h after the last administration.The levels of inflammatory factors tumor necrosis factor-α(TNF-α) and interleukin-6(IL-6) in serum,the content of malondialdehyde(MDA) and the activity of superoxide dismutase(SOD) in pancreas were detected by enzyme-linked immunosorbent assay(ELISA).Hematoxylin-eosin(HE) staining was used to observe the pathological changes of pancreatic tissue and score the pathological damage.Western blot was used to detect the expression of PI3K/Akt pathway related proteins in rat pancreas.Results The activities of serum amylase in control group,model group,positive control group,experimental-H group and experimental-H + 740Y-P group were(135.67 ± 12.89),(1 027.84 ± 32.16),(174.31 ± 15.27),(186.70 ± 17.39) and(835.92 ± 28.78)U·m L^(-1),respectively;the contents of TNF-α were(35.69 ± 3.10),(223.54 ± 15.23),(48.76 ± 4.25),(52.31 ± 4.68) and (208.46 ± 13.65) pg · m L^(-1),respectively;the contents of MDA were (2.15 ± 0.14),(6.37 ± 0.42),(2.78 ± 0.17),(2.81 ± 0.15) and (5.96 ± 0.36) nmol·mg^(-1),respectively;the histopathological injury scores were 1.12 ± 0.07,10.23 ± 0.38,3.14 ± 0.21,3.25 ± 0.23 and 9.68 ± 0.40,respectively;p-PI3K/PI3K ratios were 0.82 ± 0.05,1.96 ± 0.15,1.07 ± 0.06,1.10 ± 0.07 and 1.69 ± 0.14,respectively.The above indexes were compared with the control group in the model group,the

关 键 词：蛇床子素 磷酸肌醇3-激酶/蛋白激酶B信号通路 急性胰腺炎 

分 类 号：R28[医药卫生—中药学]