人参酚酸和人参皂苷对辐射引起小鼠肠隐窝细胞损伤的保护作用  

Protective effects of Ginseng phenolic acids and Ginsenosides on irradiation-induced injury of intestinal crypt cells in mice

作　　者：李响 石凯 梁臣 魏亮 LI Xiang;SHI Kai;LIANG Chen;WEI Liang(First Clinical School of Medicine,Xuzhou Medical University,Xuzhou 221004,Jiangsu Province,China;Cancer Biotherapy Institute of Jiangsu Province,Xuzhou Medical University,Xuzhou 221004,Jiangsu Province,China)

机构地区：[1]徐州医科大学第一临床医学院,江苏徐州221004 [2]徐州医科大学江苏省肿瘤生物治疗研究所,江苏徐州221004

出　　处：《中国临床药理学杂志》2024年第3期408-412,共5页The Chinese Journal of Clinical Pharmacology

基　　金：江苏省高等学校基础科学(自然科学)研究重大基金资助项目(22KJA320009)。

摘　　要：目的 研究人参酚酸(GPAs)和人参皂苷(Gs)在肠上皮细胞辐射损伤中的保护作用及机制。方法 将36只C57BL/6J小鼠随机分为12组,每组3只:未辐射(0 h)和辐射后4、24、48 h的模型组、酚酸组、皂苷组。模型组、酚酸组、皂苷组接受一次性10 Gy全身辐照。在辐射前48.0、24.0、0.5 h和辐射后0.5 h,酚酸组和皂苷组分别灌胃给予100 mg·kg^(-1) GPAs和100 mg·kg^(-1) Gs,模型组灌胃给予等量的0.9%NaCl。用5-溴脱氧尿核苷(BrdU)免疫组化法检测增殖的隐窝细胞,用剪切胱天蛋白酶-3(Cleaved-caspase 3)免疫组化染色法检测隐窝细胞凋亡,用免疫荧光染色法检测隐窝细胞β-连环蛋白(β-catenin)和分化抗原簇蛋白44(CD44)的表达情况。结果 在辐射后4 h,模型组、酚酸组和皂苷组的隐窝中BrdU阳性细胞分别为(487.22±113.90)、(531.56±101.19)和(714.00±84.74)个,Cleaved-caspase 3阳性细胞分别为(59.89±12.70)、(41.89±11.70)和(44.11±10.08)个,β-catenin阳性细胞分别为(522.00±69.62)、(738.56±106.24)和(707.67±117.23)个,CD44阳性细胞分别为(4.37±1.99)、(5.54±2.23)和(5.52±2.32)个。模型组的上述指标与酚酸组和皂苷组比较,在统计学上差异均有统计学意义(P<0.05,P<0.01)。结论 GPAs和Gs可抑制辐射后肠隐窝细胞凋亡,可显著提高辐射后肠隐窝细胞的增殖能力和保护肠上皮干细胞的存活,通过Wnt/β-catenin信号对放射性肠道损伤进行修复。Objective To investigate the protective effects and mechanism of ginseng phenolic acids(GPAs) and ginsenosides(Gs) on irradiation injury of intestinal epithelial cells.Methods 36 C57BL/6J mice were randomly divided into 12 groups,3 mice in each group:model group,GPAs group and Gs group at 0 h(non-radidtion)and 4 h,24 h and 48 h after radiation.The model group,GPAs group,and Gs group received a single dose of 10 Gy whole-body radiation.At 48.0,24.0,0.5 h before radiation and 0.5 h after radiation,the drug concentration of the GPAs group and the Gs group was 100 mg·kg^(-1) by gavage,the model group was given the same amount of 0.9%NaCl.The number of proliferating crypt cells was detected by 5-bromo-2'-deoxyuridine(BrdU)immunohistochemistry;Cleaved-caspase 3 immunohistochemical staining was used to detect crypt cell apoptosis;Immunofluorescence staining was used to detect the expression of β-catenin and cluster of differentiation44( CD44) in crypt cells.Results At 4 h after radiation,the Brd U positive cells of model,GPAs and Gs groups were 487.22 ± 113.90,531.56 ± 101.19 and 714.00 ± 84.74;Cleaved-caspase 3 positive cells were 59.89 ± 12.70,41.89 ± 11.70 and 44.11 ± 10.08;β-catenin positive cells were 522.00 ± 69.62,738.56 ± 106.24 and 707.67 ± 117.23;CD44positive cells were 4.37 ± 1.99,5.54 ± 2.23 and 5.52 ± 2.32.The above indexes of the model group were significantly different from those of the GPAs group and the Gs group(P < 0.05,P < 0.01).Conclusion GPAs and Gs can inhibit the apoptosis of intestinal crypt cells after radiation,significantly improve the proliferation of intestinal crypt cells and protect the survival of intestinal epithelial stem cells after radiation,and repair radiation-induced intestinal injury through Wnt/β-catenin signaling.

关 键 词：人参酚酸 人参皂苷 电离辐射 肠道损伤 肠上皮干细胞 

分 类 号：R28[医药卫生—中药学]