次声作用后大鼠延髓星形胶质细胞和神经元反应的关系  

Relationship between rats' medullary astrocytes and neurons after exposed to infrasound

作　　者：袁华[1] 龙华[1] 李玲[1] 牟翔[1] 邱建勇[1] 刘静[1] 陈景藻[1] 

机构地区：[1]第四军医大学附属西京医院理疗与康复科,陕西西安市710032

出　　处：《中国康复理论与实践》2002年第11期661-663,共3页Chinese Journal of Rehabilitation Theory and Practice

基　　金：全军"十.五"指令性课题资助项目 (01L071 )。

摘　　要：目的研究 8Hz ,90dB和 13 0dB次声作用后不同时间点 ,大鼠延髓中星形胶质细胞和神经元的反应及其相互关系。方法 8Hz ,90dB和 13 0dB次声作用于大鼠 ,2h/d ,分别作用 1、7、14、2 1、2 8天后采用免疫组织化学双标记方法 ,观察大鼠延髓中胶质源纤维酸性蛋白 (GFAP)和Fos蛋白表达的情况。结果 8Hz ,13 0dB次声作用 1d后大鼠延髓中开始出现GFAP阳性星形胶质细胞和Fos阳性神经元 ,分布相同 ,关系密切 ,并随作用次数增加而增多 ,14天后逐渐减少。 90dB组大鼠各时间点GFAP反应均比13 0dB组弱。结论 8Hz ,90dB和 13 0dB次声作用可以同时激活延髓星形胶质细胞和神经元。ObjectiveTo observe the relationship between astrocytes and neurons in rat's medulla oblongata after exposed to 8Hz, 90dB/130dB infrasound for different times. MethodsRats were exposed to 8Hz, 90dB/130dB infrasound for 2 hours per day.At the 1st,7th,12th,21th and 28th day, the expression of glial fibrillary acidic protein (GFAP) and Fos in medulla oblongata were detected using double-labling immunohistochemical method.Results GFAP-positive astrocytes and Fos-positive neurons were increased after exposure for one time. They showed similar topographic localization and formed close relationship. Their number increased as the exposure times increasing, and decreased gradually after 14 days. The reaction of 130dB group was stronger than that of 90dB group.Conclusions 8Hz, 90dB/130dB infrasound can activate both astrocytes and neurons in rats' medulla oblongata which might participate and regulate the response to infrasound.

关 键 词：次声 大鼠 延髓 星形胶质细胞 神经元反应 

分 类 号：R338[医药卫生—人体生理学] TB53[医药卫生—基础医学]