Enlarged DNA unwinding by Nme2Cas9 permits a broadened base editing window beyond the protospacer  

作　　者：Ziting Chen Xiangyang Li Qian Zhang Wenjun Sun Xiaoxuan Song Xia Zhang Xingxu Huang Bo Sun 

机构地区：[1]School of Life Science and Technology and Gene Editing Center,ShanghaiTech University,Shanghai 201210,China [2]Zhejiang Lab,Hangzhou,Zhejiang 311121,China [3]CAS Center for Excellence in Molecular Cell Science,Shanghai Institute of Biochemistry and Cell Biology,Chinese Academy of Sciences,Shanghai 200031,China [4]University of Chinese Academy of Sciences,Beijing 100049,China

出　　处：《Science China(Life Sciences)》2024年第2期424-427,共4页中国科学（生命科学英文版）

基　　金：supported by the National Natural Science Foundation of China(32022048,32271505);the Natural Science Foundation of Shanghai(22ZR1441900,23ZR1442200);the Key Research Project of Zhejiang Laboratory。

摘　　要：Dear Editor,Base editing has been developed to be one of the effective CRISPR-based gene editing techniques(Rees and Liu,2018).Base editors(BEs)take advantage of DNAmodifying enzymes fused with a catalytically impaired CRISPR protein to install base mutations.In BE,DNA-modifying enzymes often process nontarget singlestranded DNA(NTS)generated by Cas proteins.

关 键 词：INSTALL BASE WINDING 

分 类 号：Q78[生物学—分子生物学]