Characterisation of IL-21 and IL-21Rαin grass carp:IL-21-producing cells are upregulated during Flavobacterium columnare infection  

作　　者：Jiawen Xu Junya Wang Qingyu Min Wei Wang Yuting Qin Lina Lei Qian Gao Jun Zou 

机构地区：[1]Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources,Ministry of Education,Shanghai Ocean University,Shanghai,201306,China [2]International Research Center for Marine Biosciences at Shanghai Ocean University,Ministry of Science and Technology,Shanghai,201306,China [3]National Demonstration Center for Experimental Fisheries Science Education,Shanghai Ocean University,Shanghai,201306,China [4]Laboratory for Marine Biology and Biotechnology,Qingdao National Laboratory for Marine Science and Technology,Qingdao,266200,China

出　　处：《Aquaculture and Fisheries》2024年第2期126-135,共10页渔业学报（英文）

基　　金：funded by the National Natural Science Foundation of China(Grant number:32030112);National Key R&D Program of China(Grant number:2018YFD0900302).

摘　　要：Interleukin-21(IL-21)is produced mainly by activated CD4^(+)T cells and promotes the proliferation,survival and differentiation of immune cells.This study characterised a high affinity binding receptor of IL-21(IL-21 Rα)from grass carp(Ctenopharyngodon idella,Ci)and analyzed the expression of CiIL-21 and CiIL-21 Rα.It has been shown that they were modulated by PAMPs and during infection with bacterial and viral pathogens.Recombinant CiIL-21 protein produced in bacteria was active in inducing the expression of genes involved in Th responses.A monoclonal CiIL-21 antibody was generated against the rCiIL-21 and showed good reactive specificity with the rCiIL-21 proteins expressed in E.coli cells,and HEK293 T cells and the native protein from PHA-stimulated kidney leukocytes.In addition,the IL-21-producing cells detected by the CiIL-21 monoclonal antibody were found to be increased to in the kidney,spleen and hindgut after infection with Flavobacterium columnare.Our data indicate that IL-21 is involved in the regulation of immune response to bacterial and viral infection.

关 键 词：IL-21 IL-21Rα Gene expression IL-21-Producing cell Bacterial and viral infection FISH 

分 类 号：S94[农业科学—水产养殖]